Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease

A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity...

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Main Authors:	Majeed, Tanveer, Tabassum, Romana, Orts, William J., Lee, Charles C.
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886229/

id	pubmed-3886229
recordtype	oai_dc
spelling	pubmed-38862292014-01-21 Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease Majeed, Tanveer Tabassum, Romana Orts, William J. Lee, Charles C. Research Article A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8–10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. proteolyticus protease has potential applications in industries such as the detergent market. Hindawi Publishing Corporation 2013-12-25 /pmc/articles/PMC3886229/ /pubmed/24453843 http://dx.doi.org/10.1155/2013/396156 Text en Copyright © 2013 Tanveer Majeed et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Majeed, Tanveer Tabassum, Romana Orts, William J. Lee, Charles C.
spellingShingle	Majeed, Tanveer Tabassum, Romana Orts, William J. Lee, Charles C. Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
author_facet	Majeed, Tanveer Tabassum, Romana Orts, William J. Lee, Charles C.
author_sort	Majeed, Tanveer
title	Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
title_short	Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
title_full	Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
title_fullStr	Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
title_full_unstemmed	Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
title_sort	expression and characterization of coprothermobacter proteolyticus alkaline serine protease
description	A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8–10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. proteolyticus protease has potential applications in industries such as the detergent market.
publisher	Hindawi Publishing Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886229/
_version_	1612046018364309504

Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease

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