Enhancing bone marrow regeneration by SALL4 protein

Hematopoietic stem cells (HSCs) are widely used in transplantation therapy to treat a variety of blood diseases. The success of hematopoietic recovery is of high importance and closely related to the patient’s morbidity and mortality after Hematopoietic stem cell transplantation (HSCT). We have prev...

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Main Authors:	Liao, Wenbin, Aguila, Jerell R, Yao, Yixin, Yang, Jianchang, Zieve, Gary, Jiang, Yongping, Avila, Cecilia, Senzel, Lisa, Lai, Raymond, Xu, Dazhong, Dai, Wei, Ma, Yupo
Format:	Online
Language:	English
Published:	BioMed Central 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3882884/

id	pubmed-3882884
recordtype	oai_dc
spelling	pubmed-38828842014-01-08 Enhancing bone marrow regeneration by SALL4 protein Liao, Wenbin Aguila, Jerell R Yao, Yixin Yang, Jianchang Zieve, Gary Jiang, Yongping Avila, Cecilia Senzel, Lisa Lai, Raymond Xu, Dazhong Dai, Wei Ma, Yupo Research Hematopoietic stem cells (HSCs) are widely used in transplantation therapy to treat a variety of blood diseases. The success of hematopoietic recovery is of high importance and closely related to the patient’s morbidity and mortality after Hematopoietic stem cell transplantation (HSCT). We have previously shown that SALL4 is a potent stimulator for the expansion of human hematopoietic stem/progenitor cells in vitro. In these studies, we demonstrated that systemic administration with TAT-SALL4B resulted in expediting auto-reconstitution and inducing a 30-fold expansion of endogenous HSCs/HPCs in mice exposed to a high dose of irradiation. Most importantly, TAT-SALL4B treatment markedly prevented death in mice receiving lethal irradiation. Our studies also showed that TAT-SALL4B treatment was able to enhance both the short-term and long-term engraftment of human cord blood (CB) cells in NOD/SCID mice and the mechanism was likely related to the in vivo expansion of donor cells in a recipient. This robust expansion was required for the association of SALL4B with DNA methyltransferase complex, an epigenetic regulator critical in maintaining HSC pools and in normal lineage progression. Our results may provide a useful strategy to enhance hematopoietic recovery and reconstitution in cord blood transplantation with a recombinant TAT-SALL4B fusion protein. BioMed Central 2013-11-05 /pmc/articles/PMC3882884/ /pubmed/24283261 http://dx.doi.org/10.1186/1756-8722-6-84 Text en Copyright © 2013 Liao et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liao, Wenbin Aguila, Jerell R Yao, Yixin Yang, Jianchang Zieve, Gary Jiang, Yongping Avila, Cecilia Senzel, Lisa Lai, Raymond Xu, Dazhong Dai, Wei Ma, Yupo
spellingShingle	Liao, Wenbin Aguila, Jerell R Yao, Yixin Yang, Jianchang Zieve, Gary Jiang, Yongping Avila, Cecilia Senzel, Lisa Lai, Raymond Xu, Dazhong Dai, Wei Ma, Yupo Enhancing bone marrow regeneration by SALL4 protein
author_facet	Liao, Wenbin Aguila, Jerell R Yao, Yixin Yang, Jianchang Zieve, Gary Jiang, Yongping Avila, Cecilia Senzel, Lisa Lai, Raymond Xu, Dazhong Dai, Wei Ma, Yupo
author_sort	Liao, Wenbin
title	Enhancing bone marrow regeneration by SALL4 protein
title_short	Enhancing bone marrow regeneration by SALL4 protein
title_full	Enhancing bone marrow regeneration by SALL4 protein
title_fullStr	Enhancing bone marrow regeneration by SALL4 protein
title_full_unstemmed	Enhancing bone marrow regeneration by SALL4 protein
title_sort	enhancing bone marrow regeneration by sall4 protein
description	Hematopoietic stem cells (HSCs) are widely used in transplantation therapy to treat a variety of blood diseases. The success of hematopoietic recovery is of high importance and closely related to the patient’s morbidity and mortality after Hematopoietic stem cell transplantation (HSCT). We have previously shown that SALL4 is a potent stimulator for the expansion of human hematopoietic stem/progenitor cells in vitro. In these studies, we demonstrated that systemic administration with TAT-SALL4B resulted in expediting auto-reconstitution and inducing a 30-fold expansion of endogenous HSCs/HPCs in mice exposed to a high dose of irradiation. Most importantly, TAT-SALL4B treatment markedly prevented death in mice receiving lethal irradiation. Our studies also showed that TAT-SALL4B treatment was able to enhance both the short-term and long-term engraftment of human cord blood (CB) cells in NOD/SCID mice and the mechanism was likely related to the in vivo expansion of donor cells in a recipient. This robust expansion was required for the association of SALL4B with DNA methyltransferase complex, an epigenetic regulator critical in maintaining HSC pools and in normal lineage progression. Our results may provide a useful strategy to enhance hematopoietic recovery and reconstitution in cord blood transplantation with a recombinant TAT-SALL4B fusion protein.
publisher	BioMed Central
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3882884/
_version_	1612044898623553536

Enhancing bone marrow regeneration by SALL4 protein

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