A comparative study of lung toxicity in rats induced by three types of nanomaterials

The public is increasingly exposed to various engineered nanomaterials because of their mass production and wide application. Even when the biological effects of nanomaterials have been assessed, the underlying mechanisms of action in vivo are poorly understood. The present study was designed to see...

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Main Authors:	Lin, Zhiqing, Ma, Li, X, Zhu-ge, Zhang, Huashan, Lin, Bencheng
Format:	Online
Language:	English
Published:	Springer 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879061/

id	pubmed-3879061
recordtype	oai_dc
spelling	pubmed-38790612014-01-03 A comparative study of lung toxicity in rats induced by three types of nanomaterials Lin, Zhiqing Ma, Li X, Zhu-ge Zhang, Huashan Lin, Bencheng Nano Express The public is increasingly exposed to various engineered nanomaterials because of their mass production and wide application. Even when the biological effects of nanomaterials have been assessed, the underlying mechanisms of action in vivo are poorly understood. The present study was designed to seek a simple, effective, and oxidative stress-based biomarker system used for screening toxicity of nanomaterials. Nano-ferroso-ferric oxide (nano-Fe3O4), nano-silicon dioxide (nano-SiO2), and single-walled carbon nanotubes (SWCNTs) were dispersed in corn oil and characterized using transmission electron microscopy (TEM). Rats were exposed to the three nanomaterials by intratracheal instillation once every 2 days for 5 weeks. We investigated their lung oxidative and inflammatory damage by bronchoalveolar lavage fluid (BALF) detection and comparative proteomics by lung tissue. Two-dimensional electrophoresis (2-DE) of proteins isolated from the lung tissue, followed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, was performed. In the present study, we chose to detect lactate dehydrogenase, total antioxidant capacity, superoxide dismutase, and malondialdehyde as the biomarker system for screening the oxidative stress of nanomaterials and IL-6 as the inflammatory biomarker in BALF. Proteomics analysis revealed 17 differentially expressed proteins compared with the control group: nine were upregulated and eight were downregulated. Our results indicated that exposure by intratracheal instillation to any of the three typical nanomaterials may cause lung damage through oxidative damage and/or an inflammatory reaction. Springer 2013-12-09 /pmc/articles/PMC3879061/ /pubmed/24321467 http://dx.doi.org/10.1186/1556-276X-8-521 Text en Copyright © 2013 Lin et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lin, Zhiqing Ma, Li X, Zhu-ge Zhang, Huashan Lin, Bencheng
spellingShingle	Lin, Zhiqing Ma, Li X, Zhu-ge Zhang, Huashan Lin, Bencheng A comparative study of lung toxicity in rats induced by three types of nanomaterials
author_facet	Lin, Zhiqing Ma, Li X, Zhu-ge Zhang, Huashan Lin, Bencheng
author_sort	Lin, Zhiqing
title	A comparative study of lung toxicity in rats induced by three types of nanomaterials
title_short	A comparative study of lung toxicity in rats induced by three types of nanomaterials
title_full	A comparative study of lung toxicity in rats induced by three types of nanomaterials
title_fullStr	A comparative study of lung toxicity in rats induced by three types of nanomaterials
title_full_unstemmed	A comparative study of lung toxicity in rats induced by three types of nanomaterials
title_sort	comparative study of lung toxicity in rats induced by three types of nanomaterials
description	The public is increasingly exposed to various engineered nanomaterials because of their mass production and wide application. Even when the biological effects of nanomaterials have been assessed, the underlying mechanisms of action in vivo are poorly understood. The present study was designed to seek a simple, effective, and oxidative stress-based biomarker system used for screening toxicity of nanomaterials. Nano-ferroso-ferric oxide (nano-Fe3O4), nano-silicon dioxide (nano-SiO2), and single-walled carbon nanotubes (SWCNTs) were dispersed in corn oil and characterized using transmission electron microscopy (TEM). Rats were exposed to the three nanomaterials by intratracheal instillation once every 2 days for 5 weeks. We investigated their lung oxidative and inflammatory damage by bronchoalveolar lavage fluid (BALF) detection and comparative proteomics by lung tissue. Two-dimensional electrophoresis (2-DE) of proteins isolated from the lung tissue, followed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, was performed. In the present study, we chose to detect lactate dehydrogenase, total antioxidant capacity, superoxide dismutase, and malondialdehyde as the biomarker system for screening the oxidative stress of nanomaterials and IL-6 as the inflammatory biomarker in BALF. Proteomics analysis revealed 17 differentially expressed proteins compared with the control group: nine were upregulated and eight were downregulated. Our results indicated that exposure by intratracheal instillation to any of the three typical nanomaterials may cause lung damage through oxidative damage and/or an inflammatory reaction.
publisher	Springer
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879061/
_version_	1612043712471236608

A comparative study of lung toxicity in rats induced by three types of nanomaterials

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