Functional reconstitution of the mitochondrial Ca2+/H+ antiporter Letm1
The leucine zipper, EF hand–containing transmembrane protein 1 (Letm1) gene encodes a mitochondrial inner membrane protein, whose depletion severely perturbs mitochondrial Ca2+ and K+ homeostasis. Here we expressed, purified, and reconstituted human Letm1 protein in liposomes. Using Ca2+ fluorophore...
| Main Authors: | , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
The Rockefeller University Press
2014
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874562/ |