Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development

Pax7 is a member of the highly conserved Pax gene family that is expressed in restricted zones of the central nervous system (CNS) during development, being involved in early brain regionalization and the maintenance of the regional identity. Using sensitive immunohistochemical techniques we have an...

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Main Authors:	Bandín, Sandra, Morona, Ruth, Moreno, Nerea, González, Agustín
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871710/

id	pubmed-3871710
recordtype	oai_dc
spelling	pubmed-38717102014-01-07 Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development Bandín, Sandra Morona, Ruth Moreno, Nerea González, Agustín Neuroscience Pax7 is a member of the highly conserved Pax gene family that is expressed in restricted zones of the central nervous system (CNS) during development, being involved in early brain regionalization and the maintenance of the regional identity. Using sensitive immunohistochemical techniques we have analyzed the spatiotemporal pattern of Pax7 expression in the brain of the anuran amphibian Xenopus laevis, during development. Pax7 expression was first detected in early embryos in the basal plate of prosomere 3, roof and alar plates of prosomere 1 and mesencephalon, and the alar plate of rhombomere 1. As development proceeded, Pax7 cells were observed in the hypothalamus close to the catecholaminergic population of the mammillary region. In the diencephalon, Pax7 was intensely expressed in a portion of the basal plate of prosomere 3, in the roof plate and in scattered cells of the thalamus in prosomere 2, throughout the roof of prosomere 1, and in the commissural and juxtacommissural domains of the pretectum. In the mesencephalon, Pax7 cells were localized in the optic tectum and, to a lesser extent, in the torus semicircularis. The rostral portion of the alar part of rhombomere 1, including the ventricular layer of the cerebellum, expressed Pax7 and, gradually, some of these dorsal cells were observed to populate ventrally the interpeduncular nucleus and the isthmus (rhombomere 0). Additionally, Pax7 positive cells were found in the ventricular zone of the ventral part of the alar plate along the rhombencephalon and the spinal cord. The findings show that the strongly conserved features of Pax7 expression through development shared by amniote vertebrates are also present in the anamniote amphibians as a common characteristic of the brain organization of tetrapods. Frontiers Media S.A. 2013-12-24 /pmc/articles/PMC3871710/ /pubmed/24399938 http://dx.doi.org/10.3389/fnana.2013.00048 Text en Copyright © 2013 Bandín, Morona, Moreno, and González. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bandín, Sandra Morona, Ruth Moreno, Nerea González, Agustín
spellingShingle	Bandín, Sandra Morona, Ruth Moreno, Nerea González, Agustín Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development
author_facet	Bandín, Sandra Morona, Ruth Moreno, Nerea González, Agustín
author_sort	Bandín, Sandra
title	Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development
title_short	Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development
title_full	Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development
title_fullStr	Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development
title_full_unstemmed	Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development
title_sort	regional expression of pax7 in the brain of xenopus laevis during embryonic and larval development
description	Pax7 is a member of the highly conserved Pax gene family that is expressed in restricted zones of the central nervous system (CNS) during development, being involved in early brain regionalization and the maintenance of the regional identity. Using sensitive immunohistochemical techniques we have analyzed the spatiotemporal pattern of Pax7 expression in the brain of the anuran amphibian Xenopus laevis, during development. Pax7 expression was first detected in early embryos in the basal plate of prosomere 3, roof and alar plates of prosomere 1 and mesencephalon, and the alar plate of rhombomere 1. As development proceeded, Pax7 cells were observed in the hypothalamus close to the catecholaminergic population of the mammillary region. In the diencephalon, Pax7 was intensely expressed in a portion of the basal plate of prosomere 3, in the roof plate and in scattered cells of the thalamus in prosomere 2, throughout the roof of prosomere 1, and in the commissural and juxtacommissural domains of the pretectum. In the mesencephalon, Pax7 cells were localized in the optic tectum and, to a lesser extent, in the torus semicircularis. The rostral portion of the alar part of rhombomere 1, including the ventricular layer of the cerebellum, expressed Pax7 and, gradually, some of these dorsal cells were observed to populate ventrally the interpeduncular nucleus and the isthmus (rhombomere 0). Additionally, Pax7 positive cells were found in the ventricular zone of the ventral part of the alar plate along the rhombencephalon and the spinal cord. The findings show that the strongly conserved features of Pax7 expression through development shared by amniote vertebrates are also present in the anamniote amphibians as a common characteristic of the brain organization of tetrapods.
publisher	Frontiers Media S.A.
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871710/
_version_	1612041078820569088

Regional expression of Pax7 in the brain of Xenopus laevis during embryonic and larval development

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