Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma

Direct sequencing remains the most widely used method for the detection of epidermal growth factor receptor (EGFR) mutations in lung cancer; however, its relatively low sensitivity limits its clinical use. The objective of this study was to investigate the sensitivity of detecting an epidermal growt...

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Main Authors:	Kim, Hye Sook, Sung, Jae Sook, Yang, Song-Ju, Kwon, Nak-Jung, Jin, LiHua, Kim, Seung Tae, Park, Kyong Hwa, Shin, Sang Won, Kim, Han Kyeom, Kang, Jin-Hyoung, Kim, Jeong-Oh, Park, Jae Yong, Choi, Jin Eun, Yoon, HyoungKyu, Park, Chan Kwon, Yang, Kap-Seok, Seo, Jeong-Sun, Kim, Yeul Hong
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869671/

id	pubmed-3869671
recordtype	oai_dc
spelling	pubmed-38696712013-12-27 Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma Kim, Hye Sook Sung, Jae Sook Yang, Song-Ju Kwon, Nak-Jung Jin, LiHua Kim, Seung Tae Park, Kyong Hwa Shin, Sang Won Kim, Han Kyeom Kang, Jin-Hyoung Kim, Jeong-Oh Park, Jae Yong Choi, Jin Eun Yoon, HyoungKyu Park, Chan Kwon Yang, Kap-Seok Seo, Jeong-Sun Kim, Yeul Hong Research Article Direct sequencing remains the most widely used method for the detection of epidermal growth factor receptor (EGFR) mutations in lung cancer; however, its relatively low sensitivity limits its clinical use. The objective of this study was to investigate the sensitivity of detecting an epidermal growth factor receptor (EGFR) mutation from peptide nucleic acid-locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamp and Ion Torrent Personal Genome Machine (PGM) techniques compared to that by direct sequencing. Furthermore, the predictive efficacy of EGFR mutations detected by PNA-LNA PCR clamp was evaluated. EGFR mutational status was assessed by direct sequencing, PNA-LNA PCR clamp, and Ion Torrent PGM in 57 patients with non-small cell lung cancer (NSCLC). We evaluated the predictive efficacy of PNA-LNA PCR clamp on the EGFR-TKI treatment in 36 patients with advanced NSCLC retrospectively. Compared to direct sequencing (16/57, 28.1%), PNA-LNA PCR clamp (27/57, 47.4%) and Ion Torrent PGM (26/57, 45.6%) detected more EGFR mutations. EGFR mutant patients had significantly longer progressive free survival (14.31 vs. 21.61 months, P = 0.003) than that of EGFR wild patients when tested with PNA-LNA PCR clamp. However, no difference in response rate to EGFR TKIs (75.0% vs. 82.4%, P = 0.195) or overall survival (34.39 vs. 44.10 months, P = 0.422) was observed between the EGFR mutations by direct sequencing or PNA-LNA PCR clamp. Our results demonstrate firstly that patients with EGFR mutations were detected more frequently by PNA-LNA PCR clamp and Ion Torrent PGM than those by direct sequencing. EGFR mutations detected by PNA-LNA PCR clamp may be as a predicative factor for EGFR TKI response in patients with NSCLC. Public Library of Science 2013-12-20 /pmc/articles/PMC3869671/ /pubmed/24376508 http://dx.doi.org/10.1371/journal.pone.0081975 Text en © 2013 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kim, Hye Sook Sung, Jae Sook Yang, Song-Ju Kwon, Nak-Jung Jin, LiHua Kim, Seung Tae Park, Kyong Hwa Shin, Sang Won Kim, Han Kyeom Kang, Jin-Hyoung Kim, Jeong-Oh Park, Jae Yong Choi, Jin Eun Yoon, HyoungKyu Park, Chan Kwon Yang, Kap-Seok Seo, Jeong-Sun Kim, Yeul Hong
spellingShingle	Kim, Hye Sook Sung, Jae Sook Yang, Song-Ju Kwon, Nak-Jung Jin, LiHua Kim, Seung Tae Park, Kyong Hwa Shin, Sang Won Kim, Han Kyeom Kang, Jin-Hyoung Kim, Jeong-Oh Park, Jae Yong Choi, Jin Eun Yoon, HyoungKyu Park, Chan Kwon Yang, Kap-Seok Seo, Jeong-Sun Kim, Yeul Hong Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma
author_facet	Kim, Hye Sook Sung, Jae Sook Yang, Song-Ju Kwon, Nak-Jung Jin, LiHua Kim, Seung Tae Park, Kyong Hwa Shin, Sang Won Kim, Han Kyeom Kang, Jin-Hyoung Kim, Jeong-Oh Park, Jae Yong Choi, Jin Eun Yoon, HyoungKyu Park, Chan Kwon Yang, Kap-Seok Seo, Jeong-Sun Kim, Yeul Hong
author_sort	Kim, Hye Sook
title	Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma
title_short	Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma
title_full	Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma
title_fullStr	Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma
title_full_unstemmed	Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma
title_sort	predictive efficacy of low burden egfr mutation detected by next-generation sequencing on response to egfr tyrosine kinase inhibitors in non-small-cell lung carcinoma
description	Direct sequencing remains the most widely used method for the detection of epidermal growth factor receptor (EGFR) mutations in lung cancer; however, its relatively low sensitivity limits its clinical use. The objective of this study was to investigate the sensitivity of detecting an epidermal growth factor receptor (EGFR) mutation from peptide nucleic acid-locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamp and Ion Torrent Personal Genome Machine (PGM) techniques compared to that by direct sequencing. Furthermore, the predictive efficacy of EGFR mutations detected by PNA-LNA PCR clamp was evaluated. EGFR mutational status was assessed by direct sequencing, PNA-LNA PCR clamp, and Ion Torrent PGM in 57 patients with non-small cell lung cancer (NSCLC). We evaluated the predictive efficacy of PNA-LNA PCR clamp on the EGFR-TKI treatment in 36 patients with advanced NSCLC retrospectively. Compared to direct sequencing (16/57, 28.1%), PNA-LNA PCR clamp (27/57, 47.4%) and Ion Torrent PGM (26/57, 45.6%) detected more EGFR mutations. EGFR mutant patients had significantly longer progressive free survival (14.31 vs. 21.61 months, P = 0.003) than that of EGFR wild patients when tested with PNA-LNA PCR clamp. However, no difference in response rate to EGFR TKIs (75.0% vs. 82.4%, P = 0.195) or overall survival (34.39 vs. 44.10 months, P = 0.422) was observed between the EGFR mutations by direct sequencing or PNA-LNA PCR clamp. Our results demonstrate firstly that patients with EGFR mutations were detected more frequently by PNA-LNA PCR clamp and Ion Torrent PGM than those by direct sequencing. EGFR mutations detected by PNA-LNA PCR clamp may be as a predicative factor for EGFR TKI response in patients with NSCLC.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869671/
_version_	1612040377846464512

Predictive Efficacy of Low Burden EGFR Mutation Detected by Next-Generation Sequencing on Response to EGFR Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Carcinoma

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