Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells
Clonorchis sinensis (C. sinensis) has afflicted more than 35 million people in world and approximately 15 million in China, creating a socio-economic burden in epidemic regions. The infection of C. sinensis is highly related to cholangiocarcinoma and hepatocellular carcinoma (HCC). It has been docu...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Public Library of Science
2013
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868641/ |
| id |
pubmed-3868641 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-38686412013-12-23 Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells Chen, Xueqing Li, Shan He, Lei Wang, Xiaoyun Liang, Pei Chen, Wenjun Bian, Meng Ren, Mengyu Lin, Jinsi Liang, Chi Xu, Jin Wu, Zhongdao Li, Xuerong Huang, Yan Yu, Xinbing Research Article Clonorchis sinensis (C. sinensis) has afflicted more than 35 million people in world and approximately 15 million in China, creating a socio-economic burden in epidemic regions. The infection of C. sinensis is highly related to cholangiocarcinoma and hepatocellular carcinoma (HCC). It has been documented that excretory/secretory products of C. sinensis (CsESPs) involved in the pathogenesis of HCC. Csseverin, expressed at life stage of egg, metacercaria and adult worm, was a component of CsESPs. In the current study, we characterized the properties of Csseverin such as sequence signature, actin and calciumion binding activity. In addition, we demonstrated that Csseverin could cause apoptotic inhibition in spontaneously apoptotic human HCC cell line PLC cells by using morphological analysis, detection of the apoptosis-associated change of mitochondrial membrane potential (MMP) as well as Annexin V/PI apoptosis assay. Our study provided an exploratory sight view of mechanism involved in progress of carcinoma associated with the infection of C. sinensis and Csseverin might exacerbate the process of C. sinensis infected HCC patients. Public Library of Science 2013-12-19 /pmc/articles/PMC3868641/ /pubmed/24367717 http://dx.doi.org/10.1371/journal.pntd.0002606 Text en © 2013 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Chen, Xueqing Li, Shan He, Lei Wang, Xiaoyun Liang, Pei Chen, Wenjun Bian, Meng Ren, Mengyu Lin, Jinsi Liang, Chi Xu, Jin Wu, Zhongdao Li, Xuerong Huang, Yan Yu, Xinbing |
| spellingShingle |
Chen, Xueqing Li, Shan He, Lei Wang, Xiaoyun Liang, Pei Chen, Wenjun Bian, Meng Ren, Mengyu Lin, Jinsi Liang, Chi Xu, Jin Wu, Zhongdao Li, Xuerong Huang, Yan Yu, Xinbing Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells |
| author_facet |
Chen, Xueqing Li, Shan He, Lei Wang, Xiaoyun Liang, Pei Chen, Wenjun Bian, Meng Ren, Mengyu Lin, Jinsi Liang, Chi Xu, Jin Wu, Zhongdao Li, Xuerong Huang, Yan Yu, Xinbing |
| author_sort |
Chen, Xueqing |
| title |
Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells |
| title_short |
Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells |
| title_full |
Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells |
| title_fullStr |
Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells |
| title_full_unstemmed |
Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells |
| title_sort |
molecular characterization of severin from clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma plc cells |
| description |
Clonorchis sinensis (C. sinensis) has afflicted more than 35 million people in world and approximately 15 million in China, creating a socio-economic burden in epidemic regions. The infection of C. sinensis is highly related to cholangiocarcinoma and hepatocellular carcinoma (HCC). It has been documented that excretory/secretory products of C. sinensis (CsESPs) involved in the pathogenesis of HCC. Csseverin, expressed at life stage of egg, metacercaria and adult worm, was a component of CsESPs. In the current study, we characterized the properties of Csseverin such as sequence signature, actin and calciumion binding activity. In addition, we demonstrated that Csseverin could cause apoptotic inhibition in spontaneously apoptotic human HCC cell line PLC cells by using morphological analysis, detection of the apoptosis-associated change of mitochondrial membrane potential (MMP) as well as Annexin V/PI apoptosis assay. Our study provided an exploratory sight view of mechanism involved in progress of carcinoma associated with the infection of C. sinensis and Csseverin might exacerbate the process of C. sinensis infected HCC patients. |
| publisher |
Public Library of Science |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868641/ |
| _version_ |
1612040112012525568 |