Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B
Objective. We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B. Methods. The study included 241 samples, from patients suffering from systemic autoimmune d...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860079/ |
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pubmed-3860079 |
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pubmed-38600792013-12-29 Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B Infantino, M. Bentow, C. Seaman, A. Benucci, M. Atzeni, F. Sarzi-Puttini, P. Olivito, B. Meacci, F. Manfredi, M. Mahler, M. Research Article Objective. We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B. Methods. The study included 241 samples, from patients suffering from systemic autoimmune diseases (n = 108) as well as disease controls (n = 133). All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA). Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium). Results. The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0–97.7%) for anti-Ro52, 98.8% (95% CI 96.4–99.7%) for anti-Ro60, and 91.7% (95% CI 87.5–94.9%) for anti-SS-B antibodies. There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60). 30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results. Similar findings were obtained with QUANTRIX Microarray kit. Conclusion. QUANTA Flash and BioPlex 2200 show good qualitative agreement. The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies. Hindawi Publishing Corporation 2013 2013-11-27 /pmc/articles/PMC3860079/ /pubmed/24376466 http://dx.doi.org/10.1155/2013/978202 Text en Copyright © 2013 M. Infantino et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Infantino, M. Bentow, C. Seaman, A. Benucci, M. Atzeni, F. Sarzi-Puttini, P. Olivito, B. Meacci, F. Manfredi, M. Mahler, M. |
| spellingShingle |
Infantino, M. Bentow, C. Seaman, A. Benucci, M. Atzeni, F. Sarzi-Puttini, P. Olivito, B. Meacci, F. Manfredi, M. Mahler, M. Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B |
| author_facet |
Infantino, M. Bentow, C. Seaman, A. Benucci, M. Atzeni, F. Sarzi-Puttini, P. Olivito, B. Meacci, F. Manfredi, M. Mahler, M. |
| author_sort |
Infantino, M. |
| title |
Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B |
| title_short |
Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B |
| title_full |
Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B |
| title_fullStr |
Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B |
| title_full_unstemmed |
Highlights on Novel Technologies for the Detection of Antibodies to Ro60, Ro52, and SS-B |
| title_sort |
highlights on novel technologies for the detection of antibodies to ro60, ro52, and ss-b |
| description |
Objective. We aimed to compare a chemiluminescent immunoassay (CIA, QUANTA Flash) on BIO-FLASH with a multiplex flow immunoassay (MFI) on BioPlex 2200 for the detection of antibodies to Ro60, Ro52, and SS-B. Methods. The study included 241 samples, from patients suffering from systemic autoimmune diseases (n = 108) as well as disease controls (n = 133). All samples were tested for anti-Ro52, anti-Ro60, and anti-SS-B (La) antibodies on QUANTA Flash (INOVA Diagnostics, San Diego, USA) and BioPlex 2200 (Bio-Rad Laboratories Inc., Hercules, USA). Discrepant samples were tested by two independent methods: BlueDot/ANA and QUANTRIX Microarray (both D-tek, Belgium). Results. The overall qualitative agreements were 95.4% (95% confidence interval, CI 92.0–97.7%) for anti-Ro52, 98.8% (95% CI 96.4–99.7%) for anti-Ro60, and 91.7% (95% CI 87.5–94.9%) for anti-SS-B antibodies. There were 34 discrepant samples among all assays (20 anti-SS-B, 11 anti-Ro52, 3 anti-Ro60). 30/33 of retested samples (by D-tek dot blot) agreed with the QUANTA Flash results. Similar findings were obtained with QUANTRIX Microarray kit. Conclusion. QUANTA Flash and BioPlex 2200 show good qualitative agreement. The clinical performances were similar for anti-Ro52 and anti-Ro60 autoantibodies while differences were observed for anti-SS-B (La) antibodies. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860079/ |
| _version_ |
1612037650609340416 |