Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium

Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and de...

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Main Authors:	Han-Jen, Robson Ee, Wai-Fong, Yin, Kok-Gan, Chan
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859112/

id	pubmed-3859112
recordtype	oai_dc
spelling	pubmed-38591122013-12-11 Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium Han-Jen, Robson Ee Wai-Fong, Yin Kok-Gan, Chan Article Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems. Molecular Diversity Preservation International (MDPI) 2013-10-18 /pmc/articles/PMC3859112/ /pubmed/24145919 http://dx.doi.org/10.3390/s131014121 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Han-Jen, Robson Ee Wai-Fong, Yin Kok-Gan, Chan
spellingShingle	Han-Jen, Robson Ee Wai-Fong, Yin Kok-Gan, Chan Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
author_facet	Han-Jen, Robson Ee Wai-Fong, Yin Kok-Gan, Chan
author_sort	Han-Jen, Robson Ee
title	Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
title_short	Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
title_full	Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
title_fullStr	Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
title_full_unstemmed	Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium
title_sort	pandoraea sp. rb-44, a novel quorum sensing soil bacterium
description	Proteobacteria are known to communicate via signaling molecules and this process is known as quorum sensing. The most commonly studied quorum sensing molecules are N-acylhomoserine lactones (AHLs) that consists of a homoserine lactone moiety and an N-acyl side chain with various chain lengths and degrees of saturation at the C-3 position. We have isolated a bacterium, RB-44, from a site which was formally a landfill dumping ground. Using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis, this isolate was identified as a Pandoraea sp.which was then screened for AHL production using biosensors which indicated its quorum sensing properties. To identify the AHL profile of Pandoraea sp. RB-44, we used high resolution tandem mass spectrometry confirming that this isolate produced N-octanoylhomoserine lactone (C8-HSL). To the best of our knowledge, this is the first report that showed quorum sensing activity exhibited by Pandoraea sp. Our data add Pandoraea sp. to the growing number of bacteria that possess QS systems.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859112/
_version_	1612037357711654912

Pandoraea sp. RB-44, A Novel Quorum Sensing Soil Bacterium

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