Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma

Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma

Metastasis is still a major issue in cancer, and the discovery of biomarkers predicting metastatic capacity is essential for the development of better therapeutic strategies for treating lung adenocarcinoma. By using a proteomic approach, we aimed to identify novel predictors for lymph node metastas...

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Main Authors: Hayashi, Takuo, Saito, Tsuyoshi, Fujimura, Tsutomu, Hara, Kieko, Takamochi, Kazuya, Mitani, Keiko, Mineki, Reiko, Kazuno, Saiko, Oh, Shiaki, Ueno, Takashi, Suzuki, Kenji, Yao, Takashi
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858289/
id pubmed-3858289
recordtype oai_dc
spelling pubmed-38582892013-12-11 Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma Hayashi, Takuo Saito, Tsuyoshi Fujimura, Tsutomu Hara, Kieko Takamochi, Kazuya Mitani, Keiko Mineki, Reiko Kazuno, Saiko Oh, Shiaki Ueno, Takashi Suzuki, Kenji Yao, Takashi Research Article Metastasis is still a major issue in cancer, and the discovery of biomarkers predicting metastatic capacity is essential for the development of better therapeutic strategies for treating lung adenocarcinoma. By using a proteomic approach, we aimed to identify novel predictors for lymph node metastasis in lung adenocarcinoma. Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis showed 6 spots differentially expressed between lymph node metastasis-positive and lymph node metastasis-negative groups in a discovery set. Subsequent mass spectrometry showed that 2 of these spots were derived from galectin-4, and western blot analysis confirmed the overexpression of galectin-4 in metastatic samples. The predictive value of galectin-4 was confirmed by immunohistochemical analysis for a validation set consisting of 707 surgically resected specimens of lung adenocarcinomas (stages I to IV). We observed that 148 lung adenocarcinomas (20.9%) expressed galectin-4, which was significantly associated with variables of disease progression such as tumor size (p<0.0001), pleural invasion (p = 0.0071), venous invasion (p = 0.0178), nodal status (p = 0.0007), and TNM stage (p<0.0001). By the multivariate analysis, Galectin-4 expression was revealed as one of the independent predictor for lymph node metastasis, together with solid predominant and micropapillary histologic pattern. Furthermore, galectin-4 expression was revealed to be an independent predictor for lymph node metastasis and an adverse survival factor in patients with lung adenocarcinoma of acinar predominant type. Galectin-4 plays an important role in metastatic process of lung adenocarcinoma. Immunohistochemical testing for galectin-4 expression may be useful together with the detection of specific histology to predict the metastatic potential of lung adenocarcinoma. Public Library of Science 2013-12-10 /pmc/articles/PMC3858289/ /pubmed/24339976 http://dx.doi.org/10.1371/journal.pone.0081883 Text en © 2013 Hayashi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Hayashi, Takuo
Saito, Tsuyoshi
Fujimura, Tsutomu
Hara, Kieko
Takamochi, Kazuya
Mitani, Keiko
Mineki, Reiko
Kazuno, Saiko
Oh, Shiaki
Ueno, Takashi
Suzuki, Kenji
Yao, Takashi
spellingShingle Hayashi, Takuo
Saito, Tsuyoshi
Fujimura, Tsutomu
Hara, Kieko
Takamochi, Kazuya
Mitani, Keiko
Mineki, Reiko
Kazuno, Saiko
Oh, Shiaki
Ueno, Takashi
Suzuki, Kenji
Yao, Takashi
Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma
author_facet Hayashi, Takuo
Saito, Tsuyoshi
Fujimura, Tsutomu
Hara, Kieko
Takamochi, Kazuya
Mitani, Keiko
Mineki, Reiko
Kazuno, Saiko
Oh, Shiaki
Ueno, Takashi
Suzuki, Kenji
Yao, Takashi
author_sort Hayashi, Takuo
title Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma
title_short Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma
title_full Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma
title_fullStr Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma
title_full_unstemmed Galectin-4, a Novel Predictor for Lymph Node Metastasis in Lung Adenocarcinoma
title_sort galectin-4, a novel predictor for lymph node metastasis in lung adenocarcinoma
description Metastasis is still a major issue in cancer, and the discovery of biomarkers predicting metastatic capacity is essential for the development of better therapeutic strategies for treating lung adenocarcinoma. By using a proteomic approach, we aimed to identify novel predictors for lymph node metastasis in lung adenocarcinoma. Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis showed 6 spots differentially expressed between lymph node metastasis-positive and lymph node metastasis-negative groups in a discovery set. Subsequent mass spectrometry showed that 2 of these spots were derived from galectin-4, and western blot analysis confirmed the overexpression of galectin-4 in metastatic samples. The predictive value of galectin-4 was confirmed by immunohistochemical analysis for a validation set consisting of 707 surgically resected specimens of lung adenocarcinomas (stages I to IV). We observed that 148 lung adenocarcinomas (20.9%) expressed galectin-4, which was significantly associated with variables of disease progression such as tumor size (p<0.0001), pleural invasion (p = 0.0071), venous invasion (p = 0.0178), nodal status (p = 0.0007), and TNM stage (p<0.0001). By the multivariate analysis, Galectin-4 expression was revealed as one of the independent predictor for lymph node metastasis, together with solid predominant and micropapillary histologic pattern. Furthermore, galectin-4 expression was revealed to be an independent predictor for lymph node metastasis and an adverse survival factor in patients with lung adenocarcinoma of acinar predominant type. Galectin-4 plays an important role in metastatic process of lung adenocarcinoma. Immunohistochemical testing for galectin-4 expression may be useful together with the detection of specific histology to predict the metastatic potential of lung adenocarcinoma.
publisher Public Library of Science
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858289/
_version_ 1612037052152414208

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