NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis

Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis that is geographically confined to Latin America. The pro-inflammatory cytokine IL-1β that is mainly derived from the activation of the cytoplasmic multiprotein complex inflamma...

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Main Authors:	Tavares, Aldo Henrique, Magalhães, Kelly Grace, Almeida, Raquel Das Neves, Correa, Rafael, Burgel, Pedro Henrique, Bocca, Anamélia Lorenzetti
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855149/

id	pubmed-3855149
recordtype	oai_dc
spelling	pubmed-38551492013-12-11 NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis Tavares, Aldo Henrique Magalhães, Kelly Grace Almeida, Raquel Das Neves Correa, Rafael Burgel, Pedro Henrique Bocca, Anamélia Lorenzetti Research Article Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis that is geographically confined to Latin America. The pro-inflammatory cytokine IL-1β that is mainly derived from the activation of the cytoplasmic multiprotein complex inflammasome is an essential host factor against opportunistic fungal infections; however, its role in infection with a primary fungal pathogen, such as P. brasiliensis, is not well understood. In this study, we found that murine bone marrow-derived dendritic cells responded to P. brasiliensis yeast cells infection by releasing IL-1β in a spleen tyrosine kinase (Syk), caspase-1 and NOD-like receptor (NLR) family member NLRP3 dependent manner. In addition, P. brasiliensis-induced NLRP3 inflammasome activation was dependent on potassium (K+) efflux, reactive oxygen species production, phagolysosomal acidification and cathepsin B release. Finally, using mice lacking the IL-1 receptor, we demonstrated that IL-1β signaling has an important role in killing P. brasiliensis by murine macrophages. Altogether, our results demonstrate that the NLRP3 inflammasome senses and responds to P. brasiliensis yeast cells infection and plays an important role in host defense against this fungus. Public Library of Science 2013-12-05 /pmc/articles/PMC3855149/ /pubmed/24340123 http://dx.doi.org/10.1371/journal.pntd.0002595 Text en © 2013 Tavares et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tavares, Aldo Henrique Magalhães, Kelly Grace Almeida, Raquel Das Neves Correa, Rafael Burgel, Pedro Henrique Bocca, Anamélia Lorenzetti
spellingShingle	Tavares, Aldo Henrique Magalhães, Kelly Grace Almeida, Raquel Das Neves Correa, Rafael Burgel, Pedro Henrique Bocca, Anamélia Lorenzetti NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis
author_facet	Tavares, Aldo Henrique Magalhães, Kelly Grace Almeida, Raquel Das Neves Correa, Rafael Burgel, Pedro Henrique Bocca, Anamélia Lorenzetti
author_sort	Tavares, Aldo Henrique
title	NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis
title_short	NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis
title_full	NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis
title_fullStr	NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis
title_full_unstemmed	NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis
title_sort	nlrp3 inflammasome activation by paracoccidioides brasiliensis
description	Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis that is geographically confined to Latin America. The pro-inflammatory cytokine IL-1β that is mainly derived from the activation of the cytoplasmic multiprotein complex inflammasome is an essential host factor against opportunistic fungal infections; however, its role in infection with a primary fungal pathogen, such as P. brasiliensis, is not well understood. In this study, we found that murine bone marrow-derived dendritic cells responded to P. brasiliensis yeast cells infection by releasing IL-1β in a spleen tyrosine kinase (Syk), caspase-1 and NOD-like receptor (NLR) family member NLRP3 dependent manner. In addition, P. brasiliensis-induced NLRP3 inflammasome activation was dependent on potassium (K+) efflux, reactive oxygen species production, phagolysosomal acidification and cathepsin B release. Finally, using mice lacking the IL-1 receptor, we demonstrated that IL-1β signaling has an important role in killing P. brasiliensis by murine macrophages. Altogether, our results demonstrate that the NLRP3 inflammasome senses and responds to P. brasiliensis yeast cells infection and plays an important role in host defense against this fungus.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855149/
_version_	1612036125498540032

NLRP3 Inflammasome Activation by Paracoccidioides brasiliensis

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