Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets

Recently, regenerative medicine using engineered three-dimensional (3D) tissues has been focused. In the fields of cell therapy and regenerative medicine, mesenchymal stem cells (MSCs) are attractive autologous cell sources. While, in bioengineered tissues, a 3D environment may affect the differenti...

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Main Authors:	Sekine, Waki, Haraguchi, Yuji, Shimizu, Tatsuya, Yamato, Masayuki, Umezawa, Akihiro, Okano, Teruo
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852803/

id	pubmed-3852803
recordtype	oai_dc
spelling	pubmed-38528032013-12-16 Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets Sekine, Waki Haraguchi, Yuji Shimizu, Tatsuya Yamato, Masayuki Umezawa, Akihiro Okano, Teruo Research Article Recently, regenerative medicine using engineered three-dimensional (3D) tissues has been focused. In the fields of cell therapy and regenerative medicine, mesenchymal stem cells (MSCs) are attractive autologous cell sources. While, in bioengineered tissues, a 3D environment may affect the differentiation of the stem cells, little is known regarding the effect of 3D environment on cellular differentiation. In this study, MSC differentiation in in vitro 3D tissue models was assessed by human endometrial gland-derived MSCs (hEMSCs) and cell sheet technology. hEMSC sheets were layered into cell-dense 3D tissues and were cultured on porous membranes. The tissue sections revealed that chondrocyte-like cells were found within the multilayered cell sheets even at 24 h after layering. Immunostainings of chondrospecific markers were positive within those cell sheet constructs. In addition, sulfated glycosaminoglycan accumulation within the tissues increased in proportion to the numbers of layered cell sheets. The findings suggested that a high cell density and hypoxic environment in 3D tissues by layering cell sheets might accelerate a rapid differentiation of hEMSCs into chondrocytes without the help of chondro-differentiation reagents. These tissue models using cell sheets would give new insights to stem cell differentiation in 3D environment and contribute to the future application of stem cells to cartilage regenerative therapy. Hindawi Publishing Corporation 2013-11-18 /pmc/articles/PMC3852803/ /pubmed/24348153 http://dx.doi.org/10.1155/2013/359109 Text en Copyright © 2013 Waki Sekine et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sekine, Waki Haraguchi, Yuji Shimizu, Tatsuya Yamato, Masayuki Umezawa, Akihiro Okano, Teruo
spellingShingle	Sekine, Waki Haraguchi, Yuji Shimizu, Tatsuya Yamato, Masayuki Umezawa, Akihiro Okano, Teruo Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
author_facet	Sekine, Waki Haraguchi, Yuji Shimizu, Tatsuya Yamato, Masayuki Umezawa, Akihiro Okano, Teruo
author_sort	Sekine, Waki
title	Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
title_short	Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
title_full	Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
title_fullStr	Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
title_full_unstemmed	Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets
title_sort	chondrocyte differentiation of human endometrial gland-derived mscs in layered cell sheets
description	Recently, regenerative medicine using engineered three-dimensional (3D) tissues has been focused. In the fields of cell therapy and regenerative medicine, mesenchymal stem cells (MSCs) are attractive autologous cell sources. While, in bioengineered tissues, a 3D environment may affect the differentiation of the stem cells, little is known regarding the effect of 3D environment on cellular differentiation. In this study, MSC differentiation in in vitro 3D tissue models was assessed by human endometrial gland-derived MSCs (hEMSCs) and cell sheet technology. hEMSC sheets were layered into cell-dense 3D tissues and were cultured on porous membranes. The tissue sections revealed that chondrocyte-like cells were found within the multilayered cell sheets even at 24 h after layering. Immunostainings of chondrospecific markers were positive within those cell sheet constructs. In addition, sulfated glycosaminoglycan accumulation within the tissues increased in proportion to the numbers of layered cell sheets. The findings suggested that a high cell density and hypoxic environment in 3D tissues by layering cell sheets might accelerate a rapid differentiation of hEMSCs into chondrocytes without the help of chondro-differentiation reagents. These tissue models using cell sheets would give new insights to stem cell differentiation in 3D environment and contribute to the future application of stem cells to cartilage regenerative therapy.
publisher	Hindawi Publishing Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852803/
_version_	1612035235478765568

Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets

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