Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease
It has been suggested that the activation of the complement system is involved in the pathogenesis of several neurodegenerative diseases including Alzheimer’s disease (AD), Parkinson’s disease (PD), and multiple sclerosis (MS). Here, the CSF expression levels of complement proteins C3b, C4b, factor...
| Main Authors: | , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
IOS Press
2005
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851089/ |
| id |
pubmed-3851089 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-38510892013-12-22 Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease Finehout, Erin J. Franck, Zsofia Lee, Kelvin H. Other It has been suggested that the activation of the complement system is involved in the pathogenesis of several neurodegenerative diseases including Alzheimer’s disease (AD), Parkinson’s disease (PD), and multiple sclerosis (MS). Here, the CSF expression levels of complement proteins C3b, C4b, factor B, and factor H were compared between normal subjects and patients diagnosed with AD, PD, MS, and neurosyphilis. The CSF proteins were initially separated using two-dimensional gel electrophoresis, which allowed the comparison of some of the individual complement isoforms. Patients with AD, PD, and MS all showed more than one complement isoform with a significant change (p < 0.05) in CSF expression level compared to normal subjects. PD patients were found to have the greatest number of significantly changed isoforms, all showing a decreased expression level in PD CSF. The complement isoforms examined were able to distinguish between some, but not all, of the diseases studied. The data suggest that when investigating a protein as a possible biomarker, it may be useful to compare individual protein isoform expression levels in addition to the more commonly measured total protein expression level. IOS Press 2005 2005-05-26 /pmc/articles/PMC3851089/ /pubmed/15920296 http://dx.doi.org/10.1155/2005/806573 Text en Copyright © 2005 Hindawi Publishing Corporation. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Finehout, Erin J. Franck, Zsofia Lee, Kelvin H. |
| spellingShingle |
Finehout, Erin J. Franck, Zsofia Lee, Kelvin H. Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease |
| author_facet |
Finehout, Erin J. Franck, Zsofia Lee, Kelvin H. |
| author_sort |
Finehout, Erin J. |
| title |
Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease |
| title_short |
Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease |
| title_full |
Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease |
| title_fullStr |
Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease |
| title_full_unstemmed |
Complement Protein Isoforms in CSF as Possible Biomarkers for Neurodegenerative Disease |
| title_sort |
complement protein isoforms in csf as possible biomarkers for neurodegenerative disease |
| description |
It has been suggested that the activation of the complement system is involved in the pathogenesis of several neurodegenerative diseases including Alzheimer’s disease (AD), Parkinson’s disease (PD), and multiple sclerosis (MS). Here, the CSF expression levels of complement proteins C3b, C4b, factor B, and factor H were compared between normal subjects and patients diagnosed with AD, PD, MS, and neurosyphilis. The CSF proteins were initially separated using two-dimensional gel electrophoresis, which allowed the comparison of some of the individual complement isoforms. Patients with AD, PD, and MS all showed more than one complement isoform with a significant change (p < 0.05) in CSF expression level compared to normal
subjects. PD patients were found to have the greatest number of significantly changed isoforms, all showing a decreased expression
level in PD CSF. The complement isoforms examined were able to distinguish between some, but not all, of the diseases
studied. The data suggest that when investigating a protein as a possible biomarker, it may be useful to compare individual protein
isoform expression levels in addition to the more commonly measured total protein expression level. |
| publisher |
IOS Press |
| publishDate |
2005 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851089/ |
| _version_ |
1612034495025774592 |