NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
During synaptogenesis, macromolecular protein complexes assemble at the pre- and postsynaptic membrane. Extensive literature identifies numerous transmembrane molecules sufficient to induce synapse formation and several intracellular scaffolding molecules responsible for assembling active zones and...
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pubmed-38488682013-12-03 NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins Chia, Poh Hui Patel, Maulik Shen, Kang Article During synaptogenesis, macromolecular protein complexes assemble at the pre- and postsynaptic membrane. Extensive literature identifies numerous transmembrane molecules sufficient to induce synapse formation and several intracellular scaffolding molecules responsible for assembling active zones and recruiting synaptic vesicles. However, little is known about the molecular mechanisms coupling membrane receptors to active zone molecules during development. Using C.elegans, we identify an F-actin network present at nascent presynaptic terminals required for presynaptic assembly. We unravel a sequence of events where specificity-determining adhesion molecules define the location of developing synapses and locally assemble F-actin. Next, an adaptor protein NAB-1/Neurabin binds to F-actin and recruits active zone proteins, SYD-1 and SYD-2/Liprin-α by forming a tripartite complex. NAB-1 localizes transiently to synapses during development and is required for presynaptic assembly. Together, we identify a role for the actin cytoskeleton during presynaptic development and characterize a molecular pathway where NAB-1 links synaptic partner recognition to active zone assembly. 2012-01-08 /pmc/articles/PMC3848868/ /pubmed/22231427 http://dx.doi.org/10.1038/nn.2991 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
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Open Access Journal |
institution_category |
Foreign Institution |
institution |
US National Center for Biotechnology Information |
building |
NCBI PubMed |
collection |
Online Access |
language |
English |
format |
Online |
author |
Chia, Poh Hui Patel, Maulik Shen, Kang |
spellingShingle |
Chia, Poh Hui Patel, Maulik Shen, Kang NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins |
author_facet |
Chia, Poh Hui Patel, Maulik Shen, Kang |
author_sort |
Chia, Poh Hui |
title |
NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins |
title_short |
NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins |
title_full |
NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins |
title_fullStr |
NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins |
title_full_unstemmed |
NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins |
title_sort |
nab-1 instructs synapse assembly by linking adhesion molecules and f-actin to active zone proteins |
description |
During synaptogenesis, macromolecular protein complexes assemble at the pre- and postsynaptic membrane. Extensive literature identifies numerous transmembrane molecules sufficient to induce synapse formation and several intracellular scaffolding molecules responsible for assembling active zones and recruiting synaptic vesicles. However, little is known about the molecular mechanisms coupling membrane receptors to active zone molecules during development. Using C.elegans, we identify an F-actin network present at nascent presynaptic terminals required for presynaptic assembly. We unravel a sequence of events where specificity-determining adhesion molecules define the location of developing synapses and locally assemble F-actin. Next, an adaptor protein NAB-1/Neurabin binds to F-actin and recruits active zone proteins, SYD-1 and SYD-2/Liprin-α by forming a tripartite complex. NAB-1 localizes transiently to synapses during development and is required for presynaptic assembly. Together, we identify a role for the actin cytoskeleton during presynaptic development and characterize a molecular pathway where NAB-1 links synaptic partner recognition to active zone assembly. |
publishDate |
2012 |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848868/ |
_version_ |
1612033560454103040 |