NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins

During synaptogenesis, macromolecular protein complexes assemble at the pre- and postsynaptic membrane. Extensive literature identifies numerous transmembrane molecules sufficient to induce synapse formation and several intracellular scaffolding molecules responsible for assembling active zones and...

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Main Authors: Chia, Poh Hui, Patel, Maulik, Shen, Kang
Format: Online
Language:English
Published: 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848868/
id pubmed-3848868
recordtype oai_dc
spelling pubmed-38488682013-12-03 NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins Chia, Poh Hui Patel, Maulik Shen, Kang Article During synaptogenesis, macromolecular protein complexes assemble at the pre- and postsynaptic membrane. Extensive literature identifies numerous transmembrane molecules sufficient to induce synapse formation and several intracellular scaffolding molecules responsible for assembling active zones and recruiting synaptic vesicles. However, little is known about the molecular mechanisms coupling membrane receptors to active zone molecules during development. Using C.elegans, we identify an F-actin network present at nascent presynaptic terminals required for presynaptic assembly. We unravel a sequence of events where specificity-determining adhesion molecules define the location of developing synapses and locally assemble F-actin. Next, an adaptor protein NAB-1/Neurabin binds to F-actin and recruits active zone proteins, SYD-1 and SYD-2/Liprin-α by forming a tripartite complex. NAB-1 localizes transiently to synapses during development and is required for presynaptic assembly. Together, we identify a role for the actin cytoskeleton during presynaptic development and characterize a molecular pathway where NAB-1 links synaptic partner recognition to active zone assembly. 2012-01-08 /pmc/articles/PMC3848868/ /pubmed/22231427 http://dx.doi.org/10.1038/nn.2991 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Chia, Poh Hui
Patel, Maulik
Shen, Kang
spellingShingle Chia, Poh Hui
Patel, Maulik
Shen, Kang
NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
author_facet Chia, Poh Hui
Patel, Maulik
Shen, Kang
author_sort Chia, Poh Hui
title NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
title_short NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
title_full NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
title_fullStr NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
title_full_unstemmed NAB-1 instructs synapse assembly by linking adhesion molecules and F-actin to active zone proteins
title_sort nab-1 instructs synapse assembly by linking adhesion molecules and f-actin to active zone proteins
description During synaptogenesis, macromolecular protein complexes assemble at the pre- and postsynaptic membrane. Extensive literature identifies numerous transmembrane molecules sufficient to induce synapse formation and several intracellular scaffolding molecules responsible for assembling active zones and recruiting synaptic vesicles. However, little is known about the molecular mechanisms coupling membrane receptors to active zone molecules during development. Using C.elegans, we identify an F-actin network present at nascent presynaptic terminals required for presynaptic assembly. We unravel a sequence of events where specificity-determining adhesion molecules define the location of developing synapses and locally assemble F-actin. Next, an adaptor protein NAB-1/Neurabin binds to F-actin and recruits active zone proteins, SYD-1 and SYD-2/Liprin-α by forming a tripartite complex. NAB-1 localizes transiently to synapses during development and is required for presynaptic assembly. Together, we identify a role for the actin cytoskeleton during presynaptic development and characterize a molecular pathway where NAB-1 links synaptic partner recognition to active zone assembly.
publishDate 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848868/
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