Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
RNA interference (RNAi) is a powerful method used for gene expression regulation. The increasing knowledge about the molecular mechanism of this phenomenon creates new avenues for the application of the RNAi technology in the treatment of various human diseases. However, delivery of RNA interferenc...
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| Format: | Online |
| Language: | English |
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A.I. Gordeyev
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848066/ |
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pubmed-38480662013-12-03 Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs Nikitenko, N. A. Prassolov, V. S. Research Article RNA interference (RNAi) is a powerful method used for gene expression regulation. The increasing knowledge about the molecular mechanism of this phenomenon creates new avenues for the application of the RNAi technology in the treatment of various human diseases. However, delivery of RNA interference mediators, small interfering RNAs (siRNAs), to target cells is a major hurdle. Effective and safe pharmacological use of siRNAs requires carriers that can deliver siRNA to its target site and the development of methods for protection of these fragile molecules from in vivo degradation. This review summarizes various strategies for siRNA delivery, including chemical modification and non-viral approaches, such as the polymer-based, peptide-based, lipid-based techniques, and inorganic nanosystems. The advantages, disadvantages, and prospects for the therapeutic application of these methods are also examined in this paper. A.I. Gordeyev 2013 /pmc/articles/PMC3848066/ /pubmed/24303201 Text en Copyright © 2013 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Nikitenko, N. A. Prassolov, V. S. |
| spellingShingle |
Nikitenko, N. A. Prassolov, V. S. Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs |
| author_facet |
Nikitenko, N. A. Prassolov, V. S. |
| author_sort |
Nikitenko, N. A. |
| title |
Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs |
| title_short |
Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs |
| title_full |
Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs |
| title_fullStr |
Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs |
| title_full_unstemmed |
Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs |
| title_sort |
non-viral delivery and therapeutic application of small interfering rnas |
| description |
RNA interference (RNAi) is a powerful method used for gene expression
regulation. The increasing knowledge about the molecular mechanism of this
phenomenon creates new avenues for the application of the RNAi technology in
the treatment of various human diseases. However, delivery of RNA interference
mediators, small interfering RNAs (siRNAs), to target cells is a major
hurdle. Effective and safe pharmacological use of siRNAs requires carriers
that can deliver siRNA to its target site and the development of methods for
protection of these fragile molecules from in vivo degradation. This review
summarizes various strategies for siRNA delivery, including chemical
modification and non-viral approaches, such as the polymer-based,
peptide-based, lipid-based techniques, and inorganic nanosystems. The
advantages, disadvantages, and prospects for the therapeutic application of
these methods are also examined in this paper.
|
| publisher |
A.I. Gordeyev |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848066/ |
| _version_ |
1612033255611039744 |