Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro
The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethan...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844198/ |
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pubmed-3844198 |
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oai_dc |
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pubmed-38441982013-12-08 Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro Zhao, Jie Zhao, Jia-Xi Xu, Ya-Jun Research Article The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention. Hindawi Publishing Corporation 2013 2013-11-11 /pmc/articles/PMC3844198/ /pubmed/24319676 http://dx.doi.org/10.1155/2013/204187 Text en Copyright © 2013 Jie Zhao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Zhao, Jie Zhao, Jia-Xi Xu, Ya-Jun |
| spellingShingle |
Zhao, Jie Zhao, Jia-Xi Xu, Ya-Jun Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro |
| author_facet |
Zhao, Jie Zhao, Jia-Xi Xu, Ya-Jun |
| author_sort |
Zhao, Jie |
| title |
Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro
|
| title_short |
Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro
|
| title_full |
Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro
|
| title_fullStr |
Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro
|
| title_full_unstemmed |
Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol In Vitro
|
| title_sort |
exogenous nucleotides antagonize the developmental toxicity of ethanol in vitro |
| description |
The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844198/ |
| _version_ |
1612031848023588864 |