DPPrimer – A Degenerate PCR Primer Design Tool
Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of t...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Biomedical Informatics
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842581/ |
| Summary: | Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes,
from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related
or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design.
Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user
friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose
phosphorylase genes from wheat, barley and rice. |
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