High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes

We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effe...

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Main Authors:	Wu, Mengxi, Zhao, Deyao, Zhong, Wenfeng, Yan, Hao, Wang, Xiaoxia, Liang, Zicai, Li, Zhihong
Format:	Online
Language:	English
Published:	Nature Publishing Group 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842547/

id	pubmed-3842547
recordtype	oai_dc
spelling	pubmed-38425472013-12-02 High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes Wu, Mengxi Zhao, Deyao Zhong, Wenfeng Yan, Hao Wang, Xiaoxia Liang, Zicai Li, Zhihong Article We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effective for transfection of not only DNA plasmids and small interfering RNAs (siRNA), but also a small molecular anti-cancer drug, into cells in adjustable volumes of cell suspension. The method constitutes a very flexible electroporation approach in a wide range of in vitro or ex vivo scenarios in various tubes, standard multi-well plates as well as flow chambers. Nature Publishing Group 2013-11-28 /pmc/articles/PMC3842547/ /pubmed/24284649 http://dx.doi.org/10.1038/srep03370 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wu, Mengxi Zhao, Deyao Zhong, Wenfeng Yan, Hao Wang, Xiaoxia Liang, Zicai Li, Zhihong
spellingShingle	Wu, Mengxi Zhao, Deyao Zhong, Wenfeng Yan, Hao Wang, Xiaoxia Liang, Zicai Li, Zhihong High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
author_facet	Wu, Mengxi Zhao, Deyao Zhong, Wenfeng Yan, Hao Wang, Xiaoxia Liang, Zicai Li, Zhihong
author_sort	Wu, Mengxi
title	High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
title_short	High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
title_full	High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
title_fullStr	High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
title_full_unstemmed	High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
title_sort	high-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes
description	We present a multi-functional electroporation method for delivery of biomolecule utilizing a high-density distributed electrode network (HDEN) under tri-phase electric stimulation. The HDEN device, with which drastic pH change during the electroporation was avoided,was demonstrated to be highly effective for transfection of not only DNA plasmids and small interfering RNAs (siRNA), but also a small molecular anti-cancer drug, into cells in adjustable volumes of cell suspension. The method constitutes a very flexible electroporation approach in a wide range of in vitro or ex vivo scenarios in various tubes, standard multi-well plates as well as flow chambers.
publisher	Nature Publishing Group
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842547/
_version_	1612031365192089600

High-density distributed electrode network, a multi-functional electroporation method for delivery of molecules of different sizes

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