Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus

Modulation of synapses under acute stress is attracting much attention. Exposure to acute stress induces corticosterone (CORT) secretion from the adrenal cortex, resulting in rapid increase of CORT levels in plasma and the hippocampus. We tried to test whether rapid CORT effects involve activation o...

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Main Authors:	Yoshiya, Miyuki, Komatsuzaki, Yoshimasa, Hojo, Yasushi, Ikeda, Muneki, Mukai, Hideo, Hatanaka, Yusuke, Murakami, Gen, Kawata, Mitsuhiro, Kimoto, Tetsuya, Kawato, Suguru
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841935/

id	pubmed-3841935
recordtype	oai_dc
spelling	pubmed-38419352013-12-13 Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus Yoshiya, Miyuki Komatsuzaki, Yoshimasa Hojo, Yasushi Ikeda, Muneki Mukai, Hideo Hatanaka, Yusuke Murakami, Gen Kawata, Mitsuhiro Kimoto, Tetsuya Kawato, Suguru Neuroscience Modulation of synapses under acute stress is attracting much attention. Exposure to acute stress induces corticosterone (CORT) secretion from the adrenal cortex, resulting in rapid increase of CORT levels in plasma and the hippocampus. We tried to test whether rapid CORT effects involve activation of essential kinases as non-genomic processes. We demonstrated rapid effects (~1 h) of CORT on the density of thorns, by imaging Lucifer Yellow-injected neurons in adult male rat hippocampal slices. Thorns of thorny excrescences of CA3 hippocampal neurons are post-synaptic regions whose presynaptic partners are mossy fiber terminals. The application of CORT at 100, 500, and 1000 nM induced a rapid increase in the density of thorns in the stratum lucidum of CA3 pyramidal neurons. Co-administration of RU486, an antagonist of glucocorticoid receptor (GR), abolished the effect of CORT. Blocking a single kinase, including MAPK, PKA, or PKC, suppressed CORT-induced enhancement of thorn-genesis. On the other hand, GSK-3β was not involved in the signaling of thorn-genesis. Blocking AMPA receptors suppressed the CORT effect. Expression of CA3 synaptic/extranuclear GR was demonstrated by immunogold electron microscopic analysis. From these results, stress levels of CORT (100–1000 nM) might drive the rapid thorn-genesis via synaptic/extranuclear GR and multiple kinase pathways, although a role of nuclear GRs cannot be completely excluded. Frontiers Media S.A. 2013-11-27 /pmc/articles/PMC3841935/ /pubmed/24348341 http://dx.doi.org/10.3389/fncir.2013.00191 Text en Copyright © 2013 Yoshiya, Komatsuzaki, Hojo, Ikeda, Mukai, Hatanaka, Murakami, Kawata, Kimoto and Kawato. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yoshiya, Miyuki Komatsuzaki, Yoshimasa Hojo, Yasushi Ikeda, Muneki Mukai, Hideo Hatanaka, Yusuke Murakami, Gen Kawata, Mitsuhiro Kimoto, Tetsuya Kawato, Suguru
spellingShingle	Yoshiya, Miyuki Komatsuzaki, Yoshimasa Hojo, Yasushi Ikeda, Muneki Mukai, Hideo Hatanaka, Yusuke Murakami, Gen Kawata, Mitsuhiro Kimoto, Tetsuya Kawato, Suguru Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
author_facet	Yoshiya, Miyuki Komatsuzaki, Yoshimasa Hojo, Yasushi Ikeda, Muneki Mukai, Hideo Hatanaka, Yusuke Murakami, Gen Kawata, Mitsuhiro Kimoto, Tetsuya Kawato, Suguru
author_sort	Yoshiya, Miyuki
title	Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
title_short	Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
title_full	Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
title_fullStr	Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
title_full_unstemmed	Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
title_sort	corticosterone rapidly increases thorns of ca3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus
description	Modulation of synapses under acute stress is attracting much attention. Exposure to acute stress induces corticosterone (CORT) secretion from the adrenal cortex, resulting in rapid increase of CORT levels in plasma and the hippocampus. We tried to test whether rapid CORT effects involve activation of essential kinases as non-genomic processes. We demonstrated rapid effects (~1 h) of CORT on the density of thorns, by imaging Lucifer Yellow-injected neurons in adult male rat hippocampal slices. Thorns of thorny excrescences of CA3 hippocampal neurons are post-synaptic regions whose presynaptic partners are mossy fiber terminals. The application of CORT at 100, 500, and 1000 nM induced a rapid increase in the density of thorns in the stratum lucidum of CA3 pyramidal neurons. Co-administration of RU486, an antagonist of glucocorticoid receptor (GR), abolished the effect of CORT. Blocking a single kinase, including MAPK, PKA, or PKC, suppressed CORT-induced enhancement of thorn-genesis. On the other hand, GSK-3β was not involved in the signaling of thorn-genesis. Blocking AMPA receptors suppressed the CORT effect. Expression of CA3 synaptic/extranuclear GR was demonstrated by immunogold electron microscopic analysis. From these results, stress levels of CORT (100–1000 nM) might drive the rapid thorn-genesis via synaptic/extranuclear GR and multiple kinase pathways, although a role of nuclear GRs cannot be completely excluded.
publisher	Frontiers Media S.A.
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841935/
_version_	1612031172714430464

Corticosterone rapidly increases thorns of CA3 neurons via synaptic/extranuclear glucocorticoid receptor in rat hippocampus

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