Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target

The food-borne clonorchiasis imposes public health problems on inhabitants in endemic areas. Praziquantel has been employed as an efficacious anthelminthic in large-scale campaigns as well as for individual treatment of Clonorchis sinensis human infections. Although praziquantel continues to have go...

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Main Authors:	Xiao, Jing-ying, Lee, Ji-Yun, Tokuhiro, Shinji, Nagataki, Mitsuru, Jarilla, Blanca R., Nomura, Haruka, Kim, Tae Im, Hong, Sung-Jong, Agatsuma, Takeshi
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836730/

id	pubmed-3836730
recordtype	oai_dc
spelling	pubmed-38367302013-11-25 Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target Xiao, Jing-ying Lee, Ji-Yun Tokuhiro, Shinji Nagataki, Mitsuru Jarilla, Blanca R. Nomura, Haruka Kim, Tae Im Hong, Sung-Jong Agatsuma, Takeshi Research Article The food-borne clonorchiasis imposes public health problems on inhabitants in endemic areas. Praziquantel has been employed as an efficacious anthelminthic in large-scale campaigns as well as for individual treatment of Clonorchis sinensis human infections. Although praziquantel continues to have good efficacy, new drug development for this parasite has been recognized as a crucial issue to be investigated intensively. Clonorchis sinensis adults generate energy through glycolysis, actively utilizing exogenous glucose, and produce a large amount of eggs each day. Taurocyamine kinase (CsTK) is distributed abundantly in the locomotive and reproductive organs, and is an important enzyme in energy generation and homeostasis in adult C. sinensis. Enzymes of the glycolytic pathway are also expressed abundantly in these organs and in tegument, implying these organs play central roles which are essential for survival and reproduction of C. sinensis. The TK enzymes, including CsTK, are found only among invertebrate organisms and have substrate specificity for taurocyamine, which are significantly different from phosphagen kinases of vertebrate animals. With these molecular biological, enzymatic, and evolutionary characteristics, we propose here that CsTK could be a target for development of chemotherapeutic agents against C. sinensis and be a biomolecular model for other human-infecting trematodes. Public Library of Science 2013-11-21 /pmc/articles/PMC3836730/ /pubmed/24278491 http://dx.doi.org/10.1371/journal.pntd.0002548 Text en © 2013 Xiao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Xiao, Jing-ying Lee, Ji-Yun Tokuhiro, Shinji Nagataki, Mitsuru Jarilla, Blanca R. Nomura, Haruka Kim, Tae Im Hong, Sung-Jong Agatsuma, Takeshi
spellingShingle	Xiao, Jing-ying Lee, Ji-Yun Tokuhiro, Shinji Nagataki, Mitsuru Jarilla, Blanca R. Nomura, Haruka Kim, Tae Im Hong, Sung-Jong Agatsuma, Takeshi Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
author_facet	Xiao, Jing-ying Lee, Ji-Yun Tokuhiro, Shinji Nagataki, Mitsuru Jarilla, Blanca R. Nomura, Haruka Kim, Tae Im Hong, Sung-Jong Agatsuma, Takeshi
author_sort	Xiao, Jing-ying
title	Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
title_short	Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
title_full	Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
title_fullStr	Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
title_full_unstemmed	Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target
title_sort	molecular cloning and characterization of taurocyamine kinase from clonorchis sinensis: a candidate chemotherapeutic target
description	The food-borne clonorchiasis imposes public health problems on inhabitants in endemic areas. Praziquantel has been employed as an efficacious anthelminthic in large-scale campaigns as well as for individual treatment of Clonorchis sinensis human infections. Although praziquantel continues to have good efficacy, new drug development for this parasite has been recognized as a crucial issue to be investigated intensively. Clonorchis sinensis adults generate energy through glycolysis, actively utilizing exogenous glucose, and produce a large amount of eggs each day. Taurocyamine kinase (CsTK) is distributed abundantly in the locomotive and reproductive organs, and is an important enzyme in energy generation and homeostasis in adult C. sinensis. Enzymes of the glycolytic pathway are also expressed abundantly in these organs and in tegument, implying these organs play central roles which are essential for survival and reproduction of C. sinensis. The TK enzymes, including CsTK, are found only among invertebrate organisms and have substrate specificity for taurocyamine, which are significantly different from phosphagen kinases of vertebrate animals. With these molecular biological, enzymatic, and evolutionary characteristics, we propose here that CsTK could be a target for development of chemotherapeutic agents against C. sinensis and be a biomolecular model for other human-infecting trematodes.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836730/
_version_	1612029744261365760

Molecular Cloning and Characterization of Taurocyamine Kinase from Clonorchis sinensis: A Candidate Chemotherapeutic Target

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