Isolation and Quantification of MicroRNAs from Urinary Exosomes/Microvesicles for Biomarker Discovery

Recent studies indicate that microRNA (miRNA) is contained within exosome. Here we sought to optimize the methodologies for the isolation and quantification of urinary exosomal microRNA as a prelude to biomarker discovery studies. Exosomes were isolated through ultracentrifugation and characterized...

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Bibliographic Details
Main Authors: Lv, Lin-Li, Cao, Yuhan, Liu, Dan, Xu, Min, Liu, Hong, Tang, Ri-Ning, Ma, Kun-Ling, Liu, Bi-Cheng
Format: Online
Language:English
Published: Ivyspring International Publisher 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3831115/