Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells

Transplantation of allogeneic human embryonic stem cell-derived cardiac progenitors triggers an immune response. We assessed whether this response could be modulated by the concomitant use of adipose-derived stromal cells (ADSC). Peripheral blood mononuclear cells were collected from 40 patients wit...

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Main Authors:	Calderon, Damelys, Planat-Benard, Valérie, Bellamy, Valérie, Vanneaux, Valérie, Kuhn, Chantal, Peyrard, Severine, Larghero, Jéröome, Desnos, Michel, Casteilla, Louis, Pucéat, Michel, Menasché, Philippe, Chatenoud, Lucienne
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823222/

id	pubmed-3823222
recordtype	oai_dc
spelling	pubmed-38232222015-03-27 Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells Calderon, Damelys Planat-Benard, Valérie Bellamy, Valérie Vanneaux, Valérie Kuhn, Chantal Peyrard, Severine Larghero, Jéröome Desnos, Michel Casteilla, Louis Pucéat, Michel Menasché, Philippe Chatenoud, Lucienne Original Articles Transplantation of allogeneic human embryonic stem cell-derived cardiac progenitors triggers an immune response. We assessed whether this response could be modulated by the concomitant use of adipose-derived stromal cells (ADSC). Peripheral blood mononuclear cells were collected from 40 patients with coronary artery disease (CAD) and nine healthy controls. Cardiac progenitors (CD15+ Mesp1+) were generated as already reported from the I6 cell line treated with bone morphogenetic protein (BMP)-2. Adipose-derived stromal cells were obtained from abdominal dermolipectomies. We assessed the proliferative response of peripheral lymphocytes from patients and controls to cardiac progenitors cultured on a monolayer of ADSC, to allogeneic lymphocytes in mixed lymphocyte culture and to the T cell mitogen phytohemaglutin A in presence or absence of ADSC. Cardiac progenitors cultured on a monolayer of ADSC triggered a proliferation of lymphocytes from both patients and controls albeit lower than that induced by allogeneic lymphocytes. When cultured alone, ADSC did not induce any proliferation of allogeneic lymphocytes. When added to cultures of lymphocytes, ADSC significantly inhibited the alloantigen or mitogen-induced proliferative response. Compared to healthy controls, lymphocytes from patients presenting CAD expressed a decreased proliferative capacity, in particular to mitogen-induced stimulation. Adipose-derived stromal cells express an immunomodulatory effect that limits both alloantigen and mitogen-induced lymphocyte responses. Furthermore, lymphocytes from patients with CAD are low responders to conventional stimuli, possibly because of their age and disease-associated treatment regimens. We propose that, in combination, these factors may limit the in vivo immunogenicity of cardiac progenitors co-implanted with ADSC in patients with CAD. Blackwell Publishing Ltd 2012-07 2012-06-28 /pmc/articles/PMC3823222/ /pubmed/21895965 http://dx.doi.org/10.1111/j.1582-4934.2011.01435.x Text en Copyright © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Calderon, Damelys Planat-Benard, Valérie Bellamy, Valérie Vanneaux, Valérie Kuhn, Chantal Peyrard, Severine Larghero, Jéröome Desnos, Michel Casteilla, Louis Pucéat, Michel Menasché, Philippe Chatenoud, Lucienne
spellingShingle	Calderon, Damelys Planat-Benard, Valérie Bellamy, Valérie Vanneaux, Valérie Kuhn, Chantal Peyrard, Severine Larghero, Jéröome Desnos, Michel Casteilla, Louis Pucéat, Michel Menasché, Philippe Chatenoud, Lucienne Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
author_facet	Calderon, Damelys Planat-Benard, Valérie Bellamy, Valérie Vanneaux, Valérie Kuhn, Chantal Peyrard, Severine Larghero, Jéröome Desnos, Michel Casteilla, Louis Pucéat, Michel Menasché, Philippe Chatenoud, Lucienne
author_sort	Calderon, Damelys
title	Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
title_short	Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
title_full	Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
title_fullStr	Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
title_full_unstemmed	Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
title_sort	immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells
description	Transplantation of allogeneic human embryonic stem cell-derived cardiac progenitors triggers an immune response. We assessed whether this response could be modulated by the concomitant use of adipose-derived stromal cells (ADSC). Peripheral blood mononuclear cells were collected from 40 patients with coronary artery disease (CAD) and nine healthy controls. Cardiac progenitors (CD15+ Mesp1+) were generated as already reported from the I6 cell line treated with bone morphogenetic protein (BMP)-2. Adipose-derived stromal cells were obtained from abdominal dermolipectomies. We assessed the proliferative response of peripheral lymphocytes from patients and controls to cardiac progenitors cultured on a monolayer of ADSC, to allogeneic lymphocytes in mixed lymphocyte culture and to the T cell mitogen phytohemaglutin A in presence or absence of ADSC. Cardiac progenitors cultured on a monolayer of ADSC triggered a proliferation of lymphocytes from both patients and controls albeit lower than that induced by allogeneic lymphocytes. When cultured alone, ADSC did not induce any proliferation of allogeneic lymphocytes. When added to cultures of lymphocytes, ADSC significantly inhibited the alloantigen or mitogen-induced proliferative response. Compared to healthy controls, lymphocytes from patients presenting CAD expressed a decreased proliferative capacity, in particular to mitogen-induced stimulation. Adipose-derived stromal cells express an immunomodulatory effect that limits both alloantigen and mitogen-induced lymphocyte responses. Furthermore, lymphocytes from patients with CAD are low responders to conventional stimuli, possibly because of their age and disease-associated treatment regimens. We propose that, in combination, these factors may limit the in vivo immunogenicity of cardiac progenitors co-implanted with ADSC in patients with CAD.
publisher	Blackwell Publishing Ltd
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823222/
_version_	1612025292191170560

Immune response to human embryonic stem cell-derived cardiac progenitors and adipose-derived stromal cells

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