Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells

Differentiated epidermal cells can dedifferentiate into stem cells or stem cell-like cells in vivo. In this study, we report the isolation and characterization of dedifferentiation-derived cells. Epidermal sheets eliminated of basal stem cells were transplanted onto the skin wounds in 47 nude athymi...

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Main Authors:	Zhang, Cuiping, Fu, Xiaobing, Chen, Peng, Bao, Xiaoxia, Li, Fu, Sun, Xiaoyan, Lei, Yonghong, Cai, Sa, Sun, Tongzhu, Sheng, Zhiyong
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2010
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822750/

id	pubmed-3822750
recordtype	oai_dc
spelling	pubmed-38227502015-04-20 Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells Zhang, Cuiping Fu, Xiaobing Chen, Peng Bao, Xiaoxia Li, Fu Sun, Xiaoyan Lei, Yonghong Cai, Sa Sun, Tongzhu Sheng, Zhiyong Articles Differentiated epidermal cells can dedifferentiate into stem cells or stem cell-like cells in vivo. In this study, we report the isolation and characterization of dedifferentiation-derived cells. Epidermal sheets eliminated of basal stem cells were transplanted onto the skin wounds in 47 nude athymic (BALB/c-nu/nu) mice. After 5 days, cells negative for CK10 but positive for CK19 and β1-integrin emerged at the wound-neighbouring side of the epidermal sheets. Furthermore, the percentages of CK19 and β1-integrin+ cells detected by flow cytometric analysis were increased after grafting (P < 0.01) and CK10+ cells in grafted sheets decreased (P < 0.01). Then we isolated these cells on the basis of rapid adhesion to type IV collagen and found that there were 4.56% adhering cells (dedifferentiation-derived cells) in the grafting group within 10 min. The in vitro phenotypic assays showed that the expressions of CK19, β1-integrin, Oct4 and Nanog in dedifferentiation-derived cells were remarkably higher than those in the control group (differentiated epidermal cells) (P < 0.01). In addition, the results of the functional investigation of dedifferentiation-derived cells demonstrated: (1) the numbers of colonies consisting of 5–10 cells and greater than 10 cells were increased 5.9-fold and 6.7-fold, respectively, as compared with that in the control (P < 0.01); (2) more cells were in S phase and G2/M phase of the cell cycle (proliferation index values were 21.02% in control group, 45.08% in group of dedifferentiation); (3) the total days of culture (28 days versus 130 days), the passage number of cells (3 passages versus 20 passages) and assumptive total cell output (1 × 105 cells versus 1 × 1012 cells) were all significantly increased and (4) dedifferentiation-derived cells, as well as epidermal stem cells, were capable of regenerating a skin equivalent, but differentiated epidermal cells could not. These results suggested that the characteristics of dedifferentiation-derived cells cultured in vitro were similar to epidermal stem cells. This study may also offer a new approach to yield epidermal stem cells for wound repair and regeneration. Blackwell Publishing Ltd 2010-05 2009-05-01 /pmc/articles/PMC3822750/ /pubmed/19426155 http://dx.doi.org/10.1111/j.1582-4934.2009.00765.x Text en © 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhang, Cuiping Fu, Xiaobing Chen, Peng Bao, Xiaoxia Li, Fu Sun, Xiaoyan Lei, Yonghong Cai, Sa Sun, Tongzhu Sheng, Zhiyong
spellingShingle	Zhang, Cuiping Fu, Xiaobing Chen, Peng Bao, Xiaoxia Li, Fu Sun, Xiaoyan Lei, Yonghong Cai, Sa Sun, Tongzhu Sheng, Zhiyong Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
author_facet	Zhang, Cuiping Fu, Xiaobing Chen, Peng Bao, Xiaoxia Li, Fu Sun, Xiaoyan Lei, Yonghong Cai, Sa Sun, Tongzhu Sheng, Zhiyong
author_sort	Zhang, Cuiping
title	Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
title_short	Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
title_full	Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
title_fullStr	Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
title_full_unstemmed	Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
title_sort	dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
description	Differentiated epidermal cells can dedifferentiate into stem cells or stem cell-like cells in vivo. In this study, we report the isolation and characterization of dedifferentiation-derived cells. Epidermal sheets eliminated of basal stem cells were transplanted onto the skin wounds in 47 nude athymic (BALB/c-nu/nu) mice. After 5 days, cells negative for CK10 but positive for CK19 and β1-integrin emerged at the wound-neighbouring side of the epidermal sheets. Furthermore, the percentages of CK19 and β1-integrin+ cells detected by flow cytometric analysis were increased after grafting (P < 0.01) and CK10+ cells in grafted sheets decreased (P < 0.01). Then we isolated these cells on the basis of rapid adhesion to type IV collagen and found that there were 4.56% adhering cells (dedifferentiation-derived cells) in the grafting group within 10 min. The in vitro phenotypic assays showed that the expressions of CK19, β1-integrin, Oct4 and Nanog in dedifferentiation-derived cells were remarkably higher than those in the control group (differentiated epidermal cells) (P < 0.01). In addition, the results of the functional investigation of dedifferentiation-derived cells demonstrated: (1) the numbers of colonies consisting of 5–10 cells and greater than 10 cells were increased 5.9-fold and 6.7-fold, respectively, as compared with that in the control (P < 0.01); (2) more cells were in S phase and G2/M phase of the cell cycle (proliferation index values were 21.02% in control group, 45.08% in group of dedifferentiation); (3) the total days of culture (28 days versus 130 days), the passage number of cells (3 passages versus 20 passages) and assumptive total cell output (1 × 105 cells versus 1 × 1012 cells) were all significantly increased and (4) dedifferentiation-derived cells, as well as epidermal stem cells, were capable of regenerating a skin equivalent, but differentiated epidermal cells could not. These results suggested that the characteristics of dedifferentiation-derived cells cultured in vitro were similar to epidermal stem cells. This study may also offer a new approach to yield epidermal stem cells for wound repair and regeneration.
publisher	Blackwell Publishing Ltd
publishDate	2010
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822750/
_version_	1612025089289617408

Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells

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