Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency

Disturbances of iron metabolism are observed in chronic liver diseases. In the present study, we examined gene expression of duodenal iron transport molecules and hepcidin in patients with hereditary hemochromatosis (HHC) (treated and untreated), involving various genotypes (genotypes which represen...

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Main Authors:	Dostalikova-Cimburova, Marketa, Kratka, Karolina, Balusikova, Kamila, Chmelikova, Jitka, Hejda, Vaclav, Hnanicek, Jan, Neubauerova, Jitka, Vranova, Jana, Kovar, Jan, Horak, Jiri
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822694/

id	pubmed-3822694
recordtype	oai_dc
spelling	pubmed-38226942015-03-27 Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency Dostalikova-Cimburova, Marketa Kratka, Karolina Balusikova, Kamila Chmelikova, Jitka Hejda, Vaclav Hnanicek, Jan Neubauerova, Jitka Vranova, Jana Kovar, Jan Horak, Jiri Original Articles Disturbances of iron metabolism are observed in chronic liver diseases. In the present study, we examined gene expression of duodenal iron transport molecules and hepcidin in patients with hereditary hemochromatosis (HHC) (treated and untreated), involving various genotypes (genotypes which represent risk for HHC were examined), and in patients with iron deficiency anaemia (IDA). Gene expressions of DMT1, ferroportin, Dcytb, hephaestin, HFE and TFR1 were measured in duodenal biopsies using real-time PCR and Western blot. Serum hepcidin levels were measured using ELISA. DMT1, ferroportin and TFR1 mRNA levels were significantly increased in post-phlebotomized hemochromatics relative to controls. mRNAs of all tested molecules were significantly increased in patients with IDA compared to controls. The protein expression of ferroportin was increased in both groups of patients but not significantly. Spearman rank correlations showed that DMT1 versus ferroportin, Dcytb versus hephaestin and DMT1 versus TFR1 mRNAs were positively correlated regardless of the underlying cause, similarly to protein levels of ferroportin versus Dcytb and ferroportin versus hephaestin. Serum ferritin was negatively correlated with DMT1 mRNA in investigated groups of patients, except for HHC group. A decrease of serum hepcidin was observed in IDA patients, but this was not statistically significant. Our data showed that although untreated HHC patients do not have increased mRNA levels of iron transport molecules when compared to normal subjects, the expression is relatively increased in relation to body iron stores. On the other hand, post-phlebotomized HHC patients had increased DMT1 and ferroportin mRNA levels possibly due to stimulated erythropoiesis after phlebotomy. Blackwell Publishing Ltd 2012-08 2012-07-29 /pmc/articles/PMC3822694/ /pubmed/21973163 http://dx.doi.org/10.1111/j.1582-4934.2011.01458.x Text en Copyright © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Dostalikova-Cimburova, Marketa Kratka, Karolina Balusikova, Kamila Chmelikova, Jitka Hejda, Vaclav Hnanicek, Jan Neubauerova, Jitka Vranova, Jana Kovar, Jan Horak, Jiri
spellingShingle	Dostalikova-Cimburova, Marketa Kratka, Karolina Balusikova, Kamila Chmelikova, Jitka Hejda, Vaclav Hnanicek, Jan Neubauerova, Jitka Vranova, Jana Kovar, Jan Horak, Jiri Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
author_facet	Dostalikova-Cimburova, Marketa Kratka, Karolina Balusikova, Kamila Chmelikova, Jitka Hejda, Vaclav Hnanicek, Jan Neubauerova, Jitka Vranova, Jana Kovar, Jan Horak, Jiri
author_sort	Dostalikova-Cimburova, Marketa
title	Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
title_short	Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
title_full	Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
title_fullStr	Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
title_full_unstemmed	Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
title_sort	duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency
description	Disturbances of iron metabolism are observed in chronic liver diseases. In the present study, we examined gene expression of duodenal iron transport molecules and hepcidin in patients with hereditary hemochromatosis (HHC) (treated and untreated), involving various genotypes (genotypes which represent risk for HHC were examined), and in patients with iron deficiency anaemia (IDA). Gene expressions of DMT1, ferroportin, Dcytb, hephaestin, HFE and TFR1 were measured in duodenal biopsies using real-time PCR and Western blot. Serum hepcidin levels were measured using ELISA. DMT1, ferroportin and TFR1 mRNA levels were significantly increased in post-phlebotomized hemochromatics relative to controls. mRNAs of all tested molecules were significantly increased in patients with IDA compared to controls. The protein expression of ferroportin was increased in both groups of patients but not significantly. Spearman rank correlations showed that DMT1 versus ferroportin, Dcytb versus hephaestin and DMT1 versus TFR1 mRNAs were positively correlated regardless of the underlying cause, similarly to protein levels of ferroportin versus Dcytb and ferroportin versus hephaestin. Serum ferritin was negatively correlated with DMT1 mRNA in investigated groups of patients, except for HHC group. A decrease of serum hepcidin was observed in IDA patients, but this was not statistically significant. Our data showed that although untreated HHC patients do not have increased mRNA levels of iron transport molecules when compared to normal subjects, the expression is relatively increased in relation to body iron stores. On the other hand, post-phlebotomized HHC patients had increased DMT1 and ferroportin mRNA levels possibly due to stimulated erythropoiesis after phlebotomy.
publisher	Blackwell Publishing Ltd
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822694/
_version_	1612025066645618688

Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency

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