Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells

In the context of obesity, perivascular fat produces various adipokines and releases free fatty acids, which may induce inflammation and proliferation in the vascular wall. In this study we investigated how adipokines, oleic acid (OA) and the combined treatment regulate human vascular smooth muscle...

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Main Authors:	Lamers, Daniela, Schlich, Raphaela, Greulich, Sabrina, Sasson, Shlomo, Sell, Henrike, Eckel, Jürgen
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822630/

id	pubmed-3822630
recordtype	oai_dc
spelling	pubmed-38226302015-04-06 Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells Lamers, Daniela Schlich, Raphaela Greulich, Sabrina Sasson, Shlomo Sell, Henrike Eckel, Jürgen Articles In the context of obesity, perivascular fat produces various adipokines and releases free fatty acids, which may induce inflammation and proliferation in the vascular wall. In this study we investigated how adipokines, oleic acid (OA) and the combined treatment regulate human vascular smooth muscle cell (hVSMC) proliferation and migration and the underlying signalling pathways. Adipocyte-conditioned media (CM) generated from human adipocytes induces a prominent proliferation and migration of hVSMC. Autocrine action of adiponectin totally abolishes CM-induced proliferation. Furthermore, OA but not palmitic acid induces proliferation of hVSMC. CM itself does not contain fatty acids, but CM in combination with OA markedly enhances proliferation of hVSMC in a synergistic way. Both the nuclear factor (NF)-κB and the mammalian target of rapamycin (mTOR) pathway were synergistically activated under these conditions and found to be essential for hVSMC proliferation. Expression of iNOS and production of nitric oxide was only enhanced by combined treatment inducing a marked release of VEGF. Combination of OA and VEGF induces an additive increase of hVSMC proliferation. We could show that the combination of CM and OA led to a synergistic proliferation of hVSMC. Expression of iNOS and production of nitric oxide were only enhanced under these conditions and were paralleled by a marked release of VEGF. These results suggest that the combined elevated release of fatty acids and adipokines by adipose tissue in obesity might be critically related to hVSMC dysfunction, vascular inflammation and the development of atherosclerosis. Blackwell Publishing Ltd 2011-05 2010-06-01 /pmc/articles/PMC3822630/ /pubmed/20518853 http://dx.doi.org/10.1111/j.1582-4934.2010.01099.x Text en © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lamers, Daniela Schlich, Raphaela Greulich, Sabrina Sasson, Shlomo Sell, Henrike Eckel, Jürgen
spellingShingle	Lamers, Daniela Schlich, Raphaela Greulich, Sabrina Sasson, Shlomo Sell, Henrike Eckel, Jürgen Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
author_facet	Lamers, Daniela Schlich, Raphaela Greulich, Sabrina Sasson, Shlomo Sell, Henrike Eckel, Jürgen
author_sort	Lamers, Daniela
title	Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
title_short	Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
title_full	Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
title_fullStr	Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
title_full_unstemmed	Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
title_sort	oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells
description	In the context of obesity, perivascular fat produces various adipokines and releases free fatty acids, which may induce inflammation and proliferation in the vascular wall. In this study we investigated how adipokines, oleic acid (OA) and the combined treatment regulate human vascular smooth muscle cell (hVSMC) proliferation and migration and the underlying signalling pathways. Adipocyte-conditioned media (CM) generated from human adipocytes induces a prominent proliferation and migration of hVSMC. Autocrine action of adiponectin totally abolishes CM-induced proliferation. Furthermore, OA but not palmitic acid induces proliferation of hVSMC. CM itself does not contain fatty acids, but CM in combination with OA markedly enhances proliferation of hVSMC in a synergistic way. Both the nuclear factor (NF)-κB and the mammalian target of rapamycin (mTOR) pathway were synergistically activated under these conditions and found to be essential for hVSMC proliferation. Expression of iNOS and production of nitric oxide was only enhanced by combined treatment inducing a marked release of VEGF. Combination of OA and VEGF induces an additive increase of hVSMC proliferation. We could show that the combination of CM and OA led to a synergistic proliferation of hVSMC. Expression of iNOS and production of nitric oxide were only enhanced under these conditions and were paralleled by a marked release of VEGF. These results suggest that the combined elevated release of fatty acids and adipokines by adipose tissue in obesity might be critically related to hVSMC dysfunction, vascular inflammation and the development of atherosclerosis.
publisher	Blackwell Publishing Ltd
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822630/
_version_	1612025047066607616

Oleic acid and adipokines synergize in inducing proliferation and inflammatory signalling in human vascular smooth muscle cells

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