Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke

Accumulating evidence shows that post-ischemic inflammation originated by Toll-like receptors (TLR) plays critical roles in ischemic stroke. However, the functions of other innate immune receptors are poorly understood in cerebral ischemia. Macrophage-inducible C-type lectin, Mincle, is one of the i...

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Main Authors:	Suzuki, Yukiya, Nakano, Yusuke, Mishiro, Keisuke, Takagi, Toshinori, Tsuruma, Kazuhiro, Nakamura, Mitsuhiro, Yoshimura, Shinichi, Shimazawa, Masamitsu, Hara, Hideaki
Format:	Online
Language:	English
Published:	Nature Publishing Group 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822396/

id	pubmed-3822396
recordtype	oai_dc
spelling	pubmed-38223962013-11-12 Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke Suzuki, Yukiya Nakano, Yusuke Mishiro, Keisuke Takagi, Toshinori Tsuruma, Kazuhiro Nakamura, Mitsuhiro Yoshimura, Shinichi Shimazawa, Masamitsu Hara, Hideaki Article Accumulating evidence shows that post-ischemic inflammation originated by Toll-like receptors (TLR) plays critical roles in ischemic stroke. However, the functions of other innate immune receptors are poorly understood in cerebral ischemia. Macrophage-inducible C-type lectin, Mincle, is one of the innate immune receptor C-type lectin-like receptor (CLR) to response against dying cells. In the present study, we showed that Mincle, its ligand SAP130, and its downstream phospho-Syk/Syk were upregulated after ischemia, and that Mincle is expressed in immune and non-immune cells in the ischemic brains of mice and human. We treated mice with piceatannol, a Syk inhibitor, and consequently the infarct volume and swelling were suppressed by piceatannol. The levels of phospho-Syk, MMP9 and ICAM-1 were downregulated, and the level of Claudin5 was uplegurated in piceatannol-treated groups. These data indicate that innate immune system, such as Mincle and Syk plays a pivotal role in the pathogenesis after the ischemia and reperfusion. Nature Publishing Group 2013-11-11 /pmc/articles/PMC3822396/ /pubmed/24212132 http://dx.doi.org/10.1038/srep03177 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Suzuki, Yukiya Nakano, Yusuke Mishiro, Keisuke Takagi, Toshinori Tsuruma, Kazuhiro Nakamura, Mitsuhiro Yoshimura, Shinichi Shimazawa, Masamitsu Hara, Hideaki
spellingShingle	Suzuki, Yukiya Nakano, Yusuke Mishiro, Keisuke Takagi, Toshinori Tsuruma, Kazuhiro Nakamura, Mitsuhiro Yoshimura, Shinichi Shimazawa, Masamitsu Hara, Hideaki Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke
author_facet	Suzuki, Yukiya Nakano, Yusuke Mishiro, Keisuke Takagi, Toshinori Tsuruma, Kazuhiro Nakamura, Mitsuhiro Yoshimura, Shinichi Shimazawa, Masamitsu Hara, Hideaki
author_sort	Suzuki, Yukiya
title	Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke
title_short	Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke
title_full	Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke
title_fullStr	Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke
title_full_unstemmed	Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke
title_sort	involvement of mincle and syk in the changes to innate immunity after ischemic stroke
description	Accumulating evidence shows that post-ischemic inflammation originated by Toll-like receptors (TLR) plays critical roles in ischemic stroke. However, the functions of other innate immune receptors are poorly understood in cerebral ischemia. Macrophage-inducible C-type lectin, Mincle, is one of the innate immune receptor C-type lectin-like receptor (CLR) to response against dying cells. In the present study, we showed that Mincle, its ligand SAP130, and its downstream phospho-Syk/Syk were upregulated after ischemia, and that Mincle is expressed in immune and non-immune cells in the ischemic brains of mice and human. We treated mice with piceatannol, a Syk inhibitor, and consequently the infarct volume and swelling were suppressed by piceatannol. The levels of phospho-Syk, MMP9 and ICAM-1 were downregulated, and the level of Claudin5 was uplegurated in piceatannol-treated groups. These data indicate that innate immune system, such as Mincle and Syk plays a pivotal role in the pathogenesis after the ischemia and reperfusion.
publisher	Nature Publishing Group
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822396/
_version_	1612024978842058752

Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke

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