Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer
The current study investigated the effects of iodine-125 seeds on the gene methylation of SFRP2 and P16 in colorectal cancer. Mouse models of human colorectal cancer were randomly divided into an experimental group (n=25) and a control group (n=25). The control group was implanted with blank seeds (...
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D.A. Spandidos
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820808/ |
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pubmed-38208082013-11-09 Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer LI, XIAOGANG LU, PING LI, BO ZHANG, WANGFU LUO, KAIYUAN Articles The current study investigated the effects of iodine-125 seeds on the gene methylation of SFRP2 and P16 in colorectal cancer. Mouse models of human colorectal cancer were randomly divided into an experimental group (n=25) and a control group (n=25). The control group was implanted with blank seeds (0 MBq) and the experimental group with iodine-125 seeds (14.8 MBq). At 20 days after implantation, the animals were sacrificed. The methylation levels of SFRP2 and P16 were detected using methylation-specific polymerase chain reactions (MSPs). Following iodine-125 seed irradiation, the level of SFRP2 methylation decreased. The methylation index of the experimental group (0.67±0.05) was significantly lower than that of the control group (0.84±0.07; P<0.05). In the experimental group, 10 samples (40%) displayed methylation in the P16 promoter region compared with 14 (56%) in the control group, which was a significant difference (P<0.05). Iodine-125 seeds induce the downregulation of methylated tumor suppressor gene promoters, thereby inhibiting the proliferation and growth of tumor cells. D.A. Spandidos 2013-11 2013-09-13 /pmc/articles/PMC3820808/ /pubmed/24223648 http://dx.doi.org/10.3892/etm.2013.1298 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
LI, XIAOGANG LU, PING LI, BO ZHANG, WANGFU LUO, KAIYUAN |
| spellingShingle |
LI, XIAOGANG LU, PING LI, BO ZHANG, WANGFU LUO, KAIYUAN Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer |
| author_facet |
LI, XIAOGANG LU, PING LI, BO ZHANG, WANGFU LUO, KAIYUAN |
| author_sort |
LI, XIAOGANG |
| title |
Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer |
| title_short |
Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer |
| title_full |
Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer |
| title_fullStr |
Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer |
| title_full_unstemmed |
Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer |
| title_sort |
effects of iodine-125 seeds on the methylation of sfrp2 and p16 in colorectal cancer |
| description |
The current study investigated the effects of iodine-125 seeds on the gene methylation of SFRP2 and P16 in colorectal cancer. Mouse models of human colorectal cancer were randomly divided into an experimental group (n=25) and a control group (n=25). The control group was implanted with blank seeds (0 MBq) and the experimental group with iodine-125 seeds (14.8 MBq). At 20 days after implantation, the animals were sacrificed. The methylation levels of SFRP2 and P16 were detected using methylation-specific polymerase chain reactions (MSPs). Following iodine-125 seed irradiation, the level of SFRP2 methylation decreased. The methylation index of the experimental group (0.67±0.05) was significantly lower than that of the control group (0.84±0.07; P<0.05). In the experimental group, 10 samples (40%) displayed methylation in the P16 promoter region compared with 14 (56%) in the control group, which was a significant difference (P<0.05). Iodine-125 seeds induce the downregulation of methylated tumor suppressor gene promoters, thereby inhibiting the proliferation and growth of tumor cells. |
| publisher |
D.A. Spandidos |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820808/ |
| _version_ |
1612024486294454272 |