Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay

Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in...

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Main Authors:	Chantratita, Narisara, Tandhavanant, Sarunporn, Wongsuvan, Gumphol, Wuthiekanun, Vanaporn, Teerawattanasook, Nittaya, Day, Nicholas P. J., Limmathurotsakul, Direk, Peacock, Sharon J.
Format:	Online
Language:	English
Published:	The American Society of Tropical Medicine and Hygiene 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820345/

id	pubmed-3820345
recordtype	oai_dc
spelling	pubmed-38203452013-11-14 Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay Chantratita, Narisara Tandhavanant, Sarunporn Wongsuvan, Gumphol Wuthiekanun, Vanaporn Teerawattanasook, Nittaya Day, Nicholas P. J. Limmathurotsakul, Direk Peacock, Sharon J. Articles Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). The Mab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic. The American Society of Tropical Medicine and Hygiene 2013-11-06 /pmc/articles/PMC3820345/ /pubmed/24019434 http://dx.doi.org/10.4269/ajtmh.13-0212 Text en ©The American Society of Tropical Medicine and Hygiene This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chantratita, Narisara Tandhavanant, Sarunporn Wongsuvan, Gumphol Wuthiekanun, Vanaporn Teerawattanasook, Nittaya Day, Nicholas P. J. Limmathurotsakul, Direk Peacock, Sharon J.
spellingShingle	Chantratita, Narisara Tandhavanant, Sarunporn Wongsuvan, Gumphol Wuthiekanun, Vanaporn Teerawattanasook, Nittaya Day, Nicholas P. J. Limmathurotsakul, Direk Peacock, Sharon J. Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay
author_facet	Chantratita, Narisara Tandhavanant, Sarunporn Wongsuvan, Gumphol Wuthiekanun, Vanaporn Teerawattanasook, Nittaya Day, Nicholas P. J. Limmathurotsakul, Direk Peacock, Sharon J.
author_sort	Chantratita, Narisara
title	Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay
title_short	Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay
title_full	Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay
title_fullStr	Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay
title_full_unstemmed	Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay
title_sort	rapid detection of burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay
description	Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). The Mab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic.
publisher	The American Society of Tropical Medicine and Hygiene
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820345/
_version_	1612024276793163776

Rapid Detection of Burkholderia pseudomallei in Blood Cultures Using a Monoclonal Antibody-Based Immunofluorescent Assay

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