Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440

Pseudomonas putida KT2440 is a non‐pathogenic prototrophic bacterium with high potential for biotechnological applications. Despite all that is known about this strain, the biosynthesis of essential chemicals has not been fully analysed and auxotroph mutants are scarce. We carried out massive mini‐T...

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Main Authors:	Molina‐Henares, M. Antonia, García‐Salamanca, Adela, Molina‐Henares, A. Jesús, De La Torre, Jesús, Herrera, M. Carmen, Ramos, Juan L., Duque, Estrella
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2009
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815424/

id	pubmed-3815424
recordtype	oai_dc
spelling	pubmed-38154242014-02-12 Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440 Molina‐Henares, M. Antonia García‐Salamanca, Adela Molina‐Henares, A. Jesús De La Torre, Jesús Herrera, M. Carmen Ramos, Juan L. Duque, Estrella Research Articles Pseudomonas putida KT2440 is a non‐pathogenic prototrophic bacterium with high potential for biotechnological applications. Despite all that is known about this strain, the biosynthesis of essential chemicals has not been fully analysed and auxotroph mutants are scarce. We carried out massive mini‐Tn5 random mutagenesis and screened for auxotrophs that require aromatic amino acids. The biosynthesis of aromatic amino acids was analysed in detail including physical and transcriptional organization of genes, complementation assays and feeding experiments to establish pathway intermediates. There is a single pathway from chorismate leading to the biosynthesis of tryptophan, whereas the biosynthesis of phenylalanine and tyrosine is achieved through multiple convergent pathways. Genes for tryptophan biosynthesis are grouped in unlinked regions with the trpBA and trpGDE genes organized as operons and the trpI, trpE and trpF genes organized as single transcriptional units. The pheA and tyrA gene‐encoding multifunctional enzymes for phenylalanine and tyrosine biosynthesis are linked in the chromosome and form an operon with the serC gene involved in serine biosynthesis. The last step in the biosynthesis of these two amino acids requires an amino transferase activity for which multiple tyrB‐like genes are present in the host chromosome. Blackwell Publishing Ltd 2009-01 2008-12-22 /pmc/articles/PMC3815424/ /pubmed/21261884 http://dx.doi.org/10.1111/j.1751-7915.2008.00062.x Text en © 2008 The Authors; Journal compilation © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Molina‐Henares, M. Antonia García‐Salamanca, Adela Molina‐Henares, A. Jesús De La Torre, Jesús Herrera, M. Carmen Ramos, Juan L. Duque, Estrella
spellingShingle	Molina‐Henares, M. Antonia García‐Salamanca, Adela Molina‐Henares, A. Jesús De La Torre, Jesús Herrera, M. Carmen Ramos, Juan L. Duque, Estrella Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
author_facet	Molina‐Henares, M. Antonia García‐Salamanca, Adela Molina‐Henares, A. Jesús De La Torre, Jesús Herrera, M. Carmen Ramos, Juan L. Duque, Estrella
author_sort	Molina‐Henares, M. Antonia
title	Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
title_short	Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
title_full	Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
title_fullStr	Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
title_full_unstemmed	Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440
title_sort	functional analysis of aromatic biosynthetic pathways in pseudomonas putida kt2440
description	Pseudomonas putida KT2440 is a non‐pathogenic prototrophic bacterium with high potential for biotechnological applications. Despite all that is known about this strain, the biosynthesis of essential chemicals has not been fully analysed and auxotroph mutants are scarce. We carried out massive mini‐Tn5 random mutagenesis and screened for auxotrophs that require aromatic amino acids. The biosynthesis of aromatic amino acids was analysed in detail including physical and transcriptional organization of genes, complementation assays and feeding experiments to establish pathway intermediates. There is a single pathway from chorismate leading to the biosynthesis of tryptophan, whereas the biosynthesis of phenylalanine and tyrosine is achieved through multiple convergent pathways. Genes for tryptophan biosynthesis are grouped in unlinked regions with the trpBA and trpGDE genes organized as operons and the trpI, trpE and trpF genes organized as single transcriptional units. The pheA and tyrA gene‐encoding multifunctional enzymes for phenylalanine and tyrosine biosynthesis are linked in the chromosome and form an operon with the serC gene involved in serine biosynthesis. The last step in the biosynthesis of these two amino acids requires an amino transferase activity for which multiple tyrB‐like genes are present in the host chromosome.
publisher	Blackwell Publishing Ltd
publishDate	2009
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815424/
_version_	1612022812121235456

Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440

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