Plasmid addiction systems: perspectives and applications in biotechnology

Biotechnical production processes often operate with plasmid‐based expression systems in well‐established prokaryotic and eukaryotic hosts such as Escherichia coli or Saccharomyces cerevisiae, respectively. Genetically engineered organisms produce important chemicals, biopolymers, biofuels and high‐...

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Main Authors:	Kroll, Jens, Klinter, Stefan, Schneider, Cornelia, Voß, Isabella, Steinbüchel, Alexander
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2010
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815339/

id	pubmed-3815339
recordtype	oai_dc
spelling	pubmed-38153392014-02-12 Plasmid addiction systems: perspectives and applications in biotechnology Kroll, Jens Klinter, Stefan Schneider, Cornelia Voß, Isabella Steinbüchel, Alexander Minireviews Biotechnical production processes often operate with plasmid‐based expression systems in well‐established prokaryotic and eukaryotic hosts such as Escherichia coli or Saccharomyces cerevisiae, respectively. Genetically engineered organisms produce important chemicals, biopolymers, biofuels and high‐value proteins like insulin. In those bioprocesses plasmids in recombinant hosts have an essential impact on productivity. Plasmid‐free cells lead to losses in the entire product recovery and decrease the profitability of the whole process. Use of antibiotics in industrial fermentations is not an applicable option to maintain plasmid stability. Especially in pharmaceutical or GMP‐based fermentation processes, deployed antibiotics must be inactivated and removed. Several plasmid addiction systems (PAS) were described in the literature. However, not every system has reached a full applicable state. This review compares most known addiction systems and is focusing on biotechnical applications. Blackwell Publishing Ltd 2010-11 2010-04-23 /pmc/articles/PMC3815339/ /pubmed/21255361 http://dx.doi.org/10.1111/j.1751-7915.2010.00170.x Text en Copyright © 2010 The Authors. Journal compilation © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kroll, Jens Klinter, Stefan Schneider, Cornelia Voß, Isabella Steinbüchel, Alexander
spellingShingle	Kroll, Jens Klinter, Stefan Schneider, Cornelia Voß, Isabella Steinbüchel, Alexander Plasmid addiction systems: perspectives and applications in biotechnology
author_facet	Kroll, Jens Klinter, Stefan Schneider, Cornelia Voß, Isabella Steinbüchel, Alexander
author_sort	Kroll, Jens
title	Plasmid addiction systems: perspectives and applications in biotechnology
title_short	Plasmid addiction systems: perspectives and applications in biotechnology
title_full	Plasmid addiction systems: perspectives and applications in biotechnology
title_fullStr	Plasmid addiction systems: perspectives and applications in biotechnology
title_full_unstemmed	Plasmid addiction systems: perspectives and applications in biotechnology
title_sort	plasmid addiction systems: perspectives and applications in biotechnology
description	Biotechnical production processes often operate with plasmid‐based expression systems in well‐established prokaryotic and eukaryotic hosts such as Escherichia coli or Saccharomyces cerevisiae, respectively. Genetically engineered organisms produce important chemicals, biopolymers, biofuels and high‐value proteins like insulin. In those bioprocesses plasmids in recombinant hosts have an essential impact on productivity. Plasmid‐free cells lead to losses in the entire product recovery and decrease the profitability of the whole process. Use of antibiotics in industrial fermentations is not an applicable option to maintain plasmid stability. Especially in pharmaceutical or GMP‐based fermentation processes, deployed antibiotics must be inactivated and removed. Several plasmid addiction systems (PAS) were described in the literature. However, not every system has reached a full applicable state. This review compares most known addiction systems and is focusing on biotechnical applications.
publisher	Blackwell Publishing Ltd
publishDate	2010
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815339/
_version_	1612022765460652032

Plasmid addiction systems: perspectives and applications in biotechnology

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