Development of an oligonucleotide microarray method for Salmonella serotyping

Adequate identification of Salmonella enterica serovars is a prerequisite for any epidemiological investigation. This is traditionally obtained via a combination of biochemical and serological typing. However, primary strain isolation and traditional serotyping is time‐consuming and faster methods w...

Full description

Bibliographic Details
Main Authors:	Tankouo‐Sandjong, B., Sessitsch, A., Stralis‐Pavese, N., Liebana, E., Kornschober, C., Allerberger, F., Hächler, H., Bodrossy, L.
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2008
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815293/

id	pubmed-3815293
recordtype	oai_dc
spelling	pubmed-38152932014-02-12 Development of an oligonucleotide microarray method for Salmonella serotyping Tankouo‐Sandjong, B. Sessitsch, A. Stralis‐Pavese, N. Liebana, E. Kornschober, C. Allerberger, F. Hächler, H. Bodrossy, L. Research Articles Adequate identification of Salmonella enterica serovars is a prerequisite for any epidemiological investigation. This is traditionally obtained via a combination of biochemical and serological typing. However, primary strain isolation and traditional serotyping is time‐consuming and faster methods would be desirable. A microarray, based on two housekeeping and two virulence marker genes (atpD, gyrB, fliC and fljB), has been developed for the detection and identification of the two species of Salmonella (S. enterica and S. bongori), the five subspecies of S. enterica (II, IIIa, IIIb, IV, VI) and 43 S. enterica ssp. enterica serovars (covering the most prevalent ones in Austria and the UK). A comprehensive set of probes (n = 240), forming 119 probe units, was developed based on the corresponding sequences of 148 Salmonella strains, successfully validated with 57 Salmonella strains and subsequently evaluated with 35 blind samples including isolated serotypes and mixtures of different serotypes. Results demonstrated a strong discriminatory ability of the microarray among Salmonella serovars. Threshold for detection was 1 colony forming unit per 25 g of food sample following overnight (14 h) enrichment. Blackwell Publishing Ltd 2008-11 2008-10-14 /pmc/articles/PMC3815293/ /pubmed/21261872 http://dx.doi.org/10.1111/j.1751-7915.2008.00053.x Text en © 2008 The Authors. Journal compilation © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tankouo‐Sandjong, B. Sessitsch, A. Stralis‐Pavese, N. Liebana, E. Kornschober, C. Allerberger, F. Hächler, H. Bodrossy, L.
spellingShingle	Tankouo‐Sandjong, B. Sessitsch, A. Stralis‐Pavese, N. Liebana, E. Kornschober, C. Allerberger, F. Hächler, H. Bodrossy, L. Development of an oligonucleotide microarray method for Salmonella serotyping
author_facet	Tankouo‐Sandjong, B. Sessitsch, A. Stralis‐Pavese, N. Liebana, E. Kornschober, C. Allerberger, F. Hächler, H. Bodrossy, L.
author_sort	Tankouo‐Sandjong, B.
title	Development of an oligonucleotide microarray method for Salmonella serotyping
title_short	Development of an oligonucleotide microarray method for Salmonella serotyping
title_full	Development of an oligonucleotide microarray method for Salmonella serotyping
title_fullStr	Development of an oligonucleotide microarray method for Salmonella serotyping
title_full_unstemmed	Development of an oligonucleotide microarray method for Salmonella serotyping
title_sort	development of an oligonucleotide microarray method for salmonella serotyping
description	Adequate identification of Salmonella enterica serovars is a prerequisite for any epidemiological investigation. This is traditionally obtained via a combination of biochemical and serological typing. However, primary strain isolation and traditional serotyping is time‐consuming and faster methods would be desirable. A microarray, based on two housekeeping and two virulence marker genes (atpD, gyrB, fliC and fljB), has been developed for the detection and identification of the two species of Salmonella (S. enterica and S. bongori), the five subspecies of S. enterica (II, IIIa, IIIb, IV, VI) and 43 S. enterica ssp. enterica serovars (covering the most prevalent ones in Austria and the UK). A comprehensive set of probes (n = 240), forming 119 probe units, was developed based on the corresponding sequences of 148 Salmonella strains, successfully validated with 57 Salmonella strains and subsequently evaluated with 35 blind samples including isolated serotypes and mixtures of different serotypes. Results demonstrated a strong discriminatory ability of the microarray among Salmonella serovars. Threshold for detection was 1 colony forming unit per 25 g of food sample following overnight (14 h) enrichment.
publisher	Blackwell Publishing Ltd
publishDate	2008
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815293/
_version_	1612022739918389248

Development of an oligonucleotide microarray method for Salmonella serotyping

Similar Items