ABCC3 as a marker for multidrug resistance in non-small cell lung cancer

Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was...

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Main Authors:	Zhao, Yanbin, Lu, Hailing, Yan, An, Yang, Yanmei, Meng, Qingwei, Sun, Lichun, Pang, Hui, Li, Chunhong, Dong, Xiaoqun, Cai, Li
Format:	Online
Language:	English
Published:	Nature Publishing Group 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814586/

id	pubmed-3814586
recordtype	oai_dc
spelling	pubmed-38145862013-11-04 ABCC3 as a marker for multidrug resistance in non-small cell lung cancer Zhao, Yanbin Lu, Hailing Yan, An Yang, Yanmei Meng, Qingwei Sun, Lichun Pang, Hui Li, Chunhong Dong, Xiaoqun Cai, Li Article Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was done with 5 samples with highest resistance and 6 samples with highest sensitivity. Expression of ABCC3 mRNA and protein was detected by real-time PCR and immunohistochemisty, respectively. The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group. ABCC3 was one of the most up-regulated genes in the resistant group. ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS. ABCC3 expression may serve as a marker for MDR and predictor for poor clinical outcome of NSCLC. Nature Publishing Group 2013-11-01 /pmc/articles/PMC3814586/ /pubmed/24176985 http://dx.doi.org/10.1038/srep03120 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhao, Yanbin Lu, Hailing Yan, An Yang, Yanmei Meng, Qingwei Sun, Lichun Pang, Hui Li, Chunhong Dong, Xiaoqun Cai, Li
spellingShingle	Zhao, Yanbin Lu, Hailing Yan, An Yang, Yanmei Meng, Qingwei Sun, Lichun Pang, Hui Li, Chunhong Dong, Xiaoqun Cai, Li ABCC3 as a marker for multidrug resistance in non-small cell lung cancer
author_facet	Zhao, Yanbin Lu, Hailing Yan, An Yang, Yanmei Meng, Qingwei Sun, Lichun Pang, Hui Li, Chunhong Dong, Xiaoqun Cai, Li
author_sort	Zhao, Yanbin
title	ABCC3 as a marker for multidrug resistance in non-small cell lung cancer
title_short	ABCC3 as a marker for multidrug resistance in non-small cell lung cancer
title_full	ABCC3 as a marker for multidrug resistance in non-small cell lung cancer
title_fullStr	ABCC3 as a marker for multidrug resistance in non-small cell lung cancer
title_full_unstemmed	ABCC3 as a marker for multidrug resistance in non-small cell lung cancer
title_sort	abcc3 as a marker for multidrug resistance in non-small cell lung cancer
description	Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was done with 5 samples with highest resistance and 6 samples with highest sensitivity. Expression of ABCC3 mRNA and protein was detected by real-time PCR and immunohistochemisty, respectively. The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group. ABCC3 was one of the most up-regulated genes in the resistant group. ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS. ABCC3 expression may serve as a marker for MDR and predictor for poor clinical outcome of NSCLC.
publisher	Nature Publishing Group
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814586/
_version_	1612022457119539200

ABCC3 as a marker for multidrug resistance in non-small cell lung cancer

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