Effects of Prunella vulgaris on the Mice Immune Function

The present study was designed to evaluate the effects of Prunella Vulgaris (P. vulgaris) on the immune function in mice. The mice were randomly divided into one control group and three treatment groups of 10 mice each. The control group received pure water and the treatment groups received P. vulga...

Full description

Bibliographic Details
Main Authors:	Huang, Rui, Zhao, Min, Yang, Xingfen, Huang, Junming, Yang, Ying, Chen, Bifeng, Tan, Jianbin, Huang, Jiankang, Li, Zhi, Lv, Yingjian, Ji, Guiyuan
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813705/

id	pubmed-3813705
recordtype	oai_dc
spelling	pubmed-38137052013-11-07 Effects of Prunella vulgaris on the Mice Immune Function Huang, Rui Zhao, Min Yang, Xingfen Huang, Junming Yang, Ying Chen, Bifeng Tan, Jianbin Huang, Jiankang Li, Zhi Lv, Yingjian Ji, Guiyuan Research Article The present study was designed to evaluate the effects of Prunella Vulgaris (P. vulgaris) on the immune function in mice. The mice were randomly divided into one control group and three treatment groups of 10 mice each. The control group received pure water and the treatment groups received P. vulgaris extract at concentrations of 0.15, 0.30 and 0.90 g/kg BW orally for 30 days, respectively. Changes in cell immune function, non-specific immunity and humoral immunity function were evaluated. Active lymphocytes and T lymphocyte subsets were determined by fluorescence-activated cell sorting (FACS). Certain Serum concentrations of cytokines were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that, for cell immune function, compared with the control group, foot pad thickness in high dose group increased significantly (p<0.01), whereas no significant difference in the proliferative ability of splenic lymphocytes was observed among all groups (p>0.05). For non-specific immunity, NK cell activity increased significantly in a dose-dependent manner in P. vulgaris treated mice (p<0.01), mononuclear-macrophage function in medium and high dose P. vulgaris treated mice were significantly higher than that of the control group (p<0.05). For humoral immunity, no significant differences were observed in terms of the half value of hemolysis (HC50), number of hemolytic plaques and serum IgG level (p>0.05). The percentage of active T and Th lymphocytes of mice peripheral blood in high dose group were significantly higher than that of the control group (p<0.01). There was no significant difference in serum levels of IL-1β, IL-4, IL-10 and IFN-γ among all of the four groups (p>0.05). The data indicated that 0.90 g/kg BW P. vulgaris extract (equivalent to 7.5 g/kg BW crude drug) had some effect on cellular immune function and non-specific immune function in mice. Public Library of Science 2013-10-30 /pmc/articles/PMC3813705/ /pubmed/24204816 http://dx.doi.org/10.1371/journal.pone.0077355 Text en © 2013 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Huang, Rui Zhao, Min Yang, Xingfen Huang, Junming Yang, Ying Chen, Bifeng Tan, Jianbin Huang, Jiankang Li, Zhi Lv, Yingjian Ji, Guiyuan
spellingShingle	Huang, Rui Zhao, Min Yang, Xingfen Huang, Junming Yang, Ying Chen, Bifeng Tan, Jianbin Huang, Jiankang Li, Zhi Lv, Yingjian Ji, Guiyuan Effects of Prunella vulgaris on the Mice Immune Function
author_facet	Huang, Rui Zhao, Min Yang, Xingfen Huang, Junming Yang, Ying Chen, Bifeng Tan, Jianbin Huang, Jiankang Li, Zhi Lv, Yingjian Ji, Guiyuan
author_sort	Huang, Rui
title	Effects of Prunella vulgaris on the Mice Immune Function
title_short	Effects of Prunella vulgaris on the Mice Immune Function
title_full	Effects of Prunella vulgaris on the Mice Immune Function
title_fullStr	Effects of Prunella vulgaris on the Mice Immune Function
title_full_unstemmed	Effects of Prunella vulgaris on the Mice Immune Function
title_sort	effects of prunella vulgaris on the mice immune function
description	The present study was designed to evaluate the effects of Prunella Vulgaris (P. vulgaris) on the immune function in mice. The mice were randomly divided into one control group and three treatment groups of 10 mice each. The control group received pure water and the treatment groups received P. vulgaris extract at concentrations of 0.15, 0.30 and 0.90 g/kg BW orally for 30 days, respectively. Changes in cell immune function, non-specific immunity and humoral immunity function were evaluated. Active lymphocytes and T lymphocyte subsets were determined by fluorescence-activated cell sorting (FACS). Certain Serum concentrations of cytokines were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that, for cell immune function, compared with the control group, foot pad thickness in high dose group increased significantly (p<0.01), whereas no significant difference in the proliferative ability of splenic lymphocytes was observed among all groups (p>0.05). For non-specific immunity, NK cell activity increased significantly in a dose-dependent manner in P. vulgaris treated mice (p<0.01), mononuclear-macrophage function in medium and high dose P. vulgaris treated mice were significantly higher than that of the control group (p<0.05). For humoral immunity, no significant differences were observed in terms of the half value of hemolysis (HC50), number of hemolytic plaques and serum IgG level (p>0.05). The percentage of active T and Th lymphocytes of mice peripheral blood in high dose group were significantly higher than that of the control group (p<0.01). There was no significant difference in serum levels of IL-1β, IL-4, IL-10 and IFN-γ among all of the four groups (p>0.05). The data indicated that 0.90 g/kg BW P. vulgaris extract (equivalent to 7.5 g/kg BW crude drug) had some effect on cellular immune function and non-specific immune function in mice.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813705/
_version_	1612022138727825408

Effects of Prunella vulgaris on the Mice Immune Function

Similar Items