Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007
West Nile virus (WNV) appeared in the U.S. in 1999 and has since become endemic, with yearly summer epidemics causing tens of thousands of cases of serious disease over the past 14 years. Analysis of WNV strains isolated during the 2006–2007 epidemic seasons demonstrates that a new genetic variant h...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
MDPI
2013
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799518/ |
| id |
pubmed-3799518 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-37995182013-10-21 Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 Grinev, Andriyan Chancey, Caren Añez, Germán Ball, Christopher Winkelman, Valerie Williamson, Phillip Foster, Gregory A. Stramer, Susan L. Rios, Maria Article West Nile virus (WNV) appeared in the U.S. in 1999 and has since become endemic, with yearly summer epidemics causing tens of thousands of cases of serious disease over the past 14 years. Analysis of WNV strains isolated during the 2006–2007 epidemic seasons demonstrates that a new genetic variant had emerged coincidentally with an intense outbreak in Idaho during 2006. The isolates belonging to the new variant carry a 13 nt deletion, termed ID-Δ13, located at the variable region of the 3′UTR, and are genetically related. The analysis of deletions and insertions in the 3′UTR of two major lineages of WNV revealed the presence of conserved repeats and two indel motifs in the variable region of the 3′UTR. One human and two bird isolates from the Idaho 2006–2007 outbreaks were sequenced using Illumina technology and within-host variability was analyzed. Continued monitoring of new genetic variants is important for public health as WNV continues to evolve. MDPI 2013-09-23 2013-09 /pmc/articles/PMC3799518/ /pubmed/24065039 http://dx.doi.org/10.3390/ijerph10094486 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Grinev, Andriyan Chancey, Caren Añez, Germán Ball, Christopher Winkelman, Valerie Williamson, Phillip Foster, Gregory A. Stramer, Susan L. Rios, Maria |
| spellingShingle |
Grinev, Andriyan Chancey, Caren Añez, Germán Ball, Christopher Winkelman, Valerie Williamson, Phillip Foster, Gregory A. Stramer, Susan L. Rios, Maria Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 |
| author_facet |
Grinev, Andriyan Chancey, Caren Añez, Germán Ball, Christopher Winkelman, Valerie Williamson, Phillip Foster, Gregory A. Stramer, Susan L. Rios, Maria |
| author_sort |
Grinev, Andriyan |
| title |
Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 |
| title_short |
Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 |
| title_full |
Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 |
| title_fullStr |
Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 |
| title_full_unstemmed |
Genetic Analysis of West Nile Virus Isolates from an Outbreak in Idaho, United States, 2006–2007 |
| title_sort |
genetic analysis of west nile virus isolates from an outbreak in idaho, united states, 2006–2007 |
| description |
West Nile virus (WNV) appeared in the U.S. in 1999 and has since become endemic, with yearly summer epidemics causing tens of thousands of cases of serious disease over the past 14 years. Analysis of WNV strains isolated during the 2006–2007 epidemic seasons demonstrates that a new genetic variant had emerged coincidentally with an intense outbreak in Idaho during 2006. The isolates belonging to the new variant carry a 13 nt deletion, termed ID-Δ13, located at the variable region of the 3′UTR, and are genetically related. The analysis of deletions and insertions in the 3′UTR of two major lineages of WNV revealed the presence of conserved repeats and two indel motifs in the variable region of the 3′UTR. One human and two bird isolates from the Idaho 2006–2007 outbreaks were sequenced using Illumina technology and within-host variability was analyzed. Continued monitoring of new genetic variants is important for public health as WNV continues to evolve. |
| publisher |
MDPI |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799518/ |
| _version_ |
1612019118460895232 |