Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes

Recent success in the derivation of haploid embryonic stem cells (haESCs) from mouse via parthenogenesis and androgenesis has enabled genetic screening in mammalian cells and generation of gene-modified animals. However, whether haESCs can be derived from primates remains unknown. Here, we report th...

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Main Authors:	Yang, Hui, Liu, Zhen, Ma, Yu, Zhong, Cuiqing, Yin, Qi, Zhou, Chikai, Shi, Linyu, Cai, Yijun, Zhao, Hanzhi, Wang, Hui, Tang, Fan, Wang, Yan, Zhang, Chenchen, Liu, Xin-yuan, Lai, Dongmei, Jin, Ying, Sun, Qiang, Li, Jinsong
Format:	Online
Language:	English
Published:	Nature Publishing Group 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790242/

id	pubmed-3790242
recordtype	oai_dc
spelling	pubmed-37902422013-10-18 Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes Yang, Hui Liu, Zhen Ma, Yu Zhong, Cuiqing Yin, Qi Zhou, Chikai Shi, Linyu Cai, Yijun Zhao, Hanzhi Wang, Hui Tang, Fan Wang, Yan Zhang, Chenchen Liu, Xin-yuan Lai, Dongmei Jin, Ying Sun, Qiang Li, Jinsong Original Article Recent success in the derivation of haploid embryonic stem cells (haESCs) from mouse via parthenogenesis and androgenesis has enabled genetic screening in mammalian cells and generation of gene-modified animals. However, whether haESCs can be derived from primates remains unknown. Here, we report the derivation of haESCs from parthenogenetic blastocysts of Macaca fascicularis monkeys. These cells, termed as PG-haESCs, are pluripotent and can differentiate to cells of three embryonic germ layers in vitro or in vivo. Interestingly, the haploidy of one monkey PG-haESC line (MPH1) is more stable compared with that of the other one (MPH2), as shown by the existence of haploid cells for more than 140 days without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Importantly, transgenic monkey PG-haESC lines can be generated by lentivirus- and piggyBac transposon-mediated gene transfer. Moreover, genetic screening is feasible in monkey PG-haESCs. Our results demonstrate that PG-haESCs can be generated from monkeys, providing an ideal tool for genetic analyses in primates. Nature Publishing Group 2013-10 2013-07-16 /pmc/articles/PMC3790242/ /pubmed/23856644 http://dx.doi.org/10.1038/cr.2013.93 Text en Copyright © 2013 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yang, Hui Liu, Zhen Ma, Yu Zhong, Cuiqing Yin, Qi Zhou, Chikai Shi, Linyu Cai, Yijun Zhao, Hanzhi Wang, Hui Tang, Fan Wang, Yan Zhang, Chenchen Liu, Xin-yuan Lai, Dongmei Jin, Ying Sun, Qiang Li, Jinsong
spellingShingle	Yang, Hui Liu, Zhen Ma, Yu Zhong, Cuiqing Yin, Qi Zhou, Chikai Shi, Linyu Cai, Yijun Zhao, Hanzhi Wang, Hui Tang, Fan Wang, Yan Zhang, Chenchen Liu, Xin-yuan Lai, Dongmei Jin, Ying Sun, Qiang Li, Jinsong Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
author_facet	Yang, Hui Liu, Zhen Ma, Yu Zhong, Cuiqing Yin, Qi Zhou, Chikai Shi, Linyu Cai, Yijun Zhao, Hanzhi Wang, Hui Tang, Fan Wang, Yan Zhang, Chenchen Liu, Xin-yuan Lai, Dongmei Jin, Ying Sun, Qiang Li, Jinsong
author_sort	Yang, Hui
title	Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
title_short	Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
title_full	Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
title_fullStr	Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
title_full_unstemmed	Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
title_sort	generation of haploid embryonic stem cells from macaca fascicularis monkey parthenotes
description	Recent success in the derivation of haploid embryonic stem cells (haESCs) from mouse via parthenogenesis and androgenesis has enabled genetic screening in mammalian cells and generation of gene-modified animals. However, whether haESCs can be derived from primates remains unknown. Here, we report the derivation of haESCs from parthenogenetic blastocysts of Macaca fascicularis monkeys. These cells, termed as PG-haESCs, are pluripotent and can differentiate to cells of three embryonic germ layers in vitro or in vivo. Interestingly, the haploidy of one monkey PG-haESC line (MPH1) is more stable compared with that of the other one (MPH2), as shown by the existence of haploid cells for more than 140 days without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Importantly, transgenic monkey PG-haESC lines can be generated by lentivirus- and piggyBac transposon-mediated gene transfer. Moreover, genetic screening is feasible in monkey PG-haESCs. Our results demonstrate that PG-haESCs can be generated from monkeys, providing an ideal tool for genetic analyses in primates.
publisher	Nature Publishing Group
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790242/
_version_	1612016253695688704

Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes

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