Targeted Mutagenesis of Arabidopsis thaliana Using Engineered TAL Effector Nucleases

Custom TAL effector nucleases (TALENs) are increasingly used as reagents to manipulate genomes in vivo. Here, we used TALENs to modify the genome of the model plant, Arabidopsis thaliana. We engineered seven TALENs targeting five Arabidopsis genes, namely ADH1, TT4, MAPKKK1, DSK2B, and NATA2. In poo...

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Bibliographic Details
Main Authors: Christian, Michelle, Qi, Yiping, Zhang, Yong, Voytas, Daniel F.
Format: Online
Language:English
Published: Genetics Society of America 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789794/