Improved Detection of Rare HIV-1 Variants using 454 Pyrosequencing

Improved Detection of Rare HIV-1 Variants using 454 Pyrosequencing

454 pyrosequencing, a massively parallel sequencing (MPS) technology, is often used to study HIV genetic variation. However, the substantial mismatch error rate of the PCR required to prepare HIV-containing samples for pyrosequencing has limited the detection of rare variants within viral population...

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Bibliographic Details
Main Authors: Larsen, Brendan B., Chen, Lennie, Maust, Brandon S., Kim, Moon, Zhao, Hong, Deng, Wenjie, Westfall, Dylan, Beck, Ingrid, Frenkel, Lisa M., Mullins, James I.
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788733/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788733/

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