Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate

The surface plasmon resonance (SPR) technique is a well-known optical method that can be used to measure the refractive index of organic nano-layers adsorbed on a thin metal film. Although there are many configurations for measuring biomolecular interactions, SPR-based techniques play a central role...

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Main Authors:	Parisi, Antonino, Cino, Alfonso C., Busacca, Alessandro C., Cherchi, Matteo, Riva-Sanseverino, Stefano
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2008
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787434/

id	pubmed-3787434
recordtype	oai_dc
spelling	pubmed-37874342013-10-17 Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate Parisi, Antonino Cino, Alfonso C. Busacca, Alessandro C. Cherchi, Matteo Riva-Sanseverino, Stefano Article The surface plasmon resonance (SPR) technique is a well-known optical method that can be used to measure the refractive index of organic nano-layers adsorbed on a thin metal film. Although there are many configurations for measuring biomolecular interactions, SPR-based techniques play a central role in many current biosensing experiments, since they are the most suited for sensitive and quantitative kinetic measurements. Here we give some results from the analysis and numerical elaboration of SPR data from integrated optics experiments in a particular borosilicate glass, chosen for its composition offering the rather low refractive index of 1.4701 at 633 nm wavelength. These data regard the flow over the sensing region (metal window) of different solutions with refractive indexes in the range of interest (1.3÷1.5) for the detection of contaminants in aqueous solutions. After a discussion of the principles of SPR, of the metal window design optimization by means of optical interaction numerical modeling, and of waveguide fabrication techniques, we give a description of system setup and experimental results. Optimum gold film window thickness and width in this guided-wave configuration has been for the first time derived and implemented on an integrated optic prototype device. Its characterization is given by means of the real time waveguide output intensity measurements, which correspond to the interaction between the sensing gold thin film window and the flowing analyte. The SPR curve was subsequently inferred. Finally, a modified version of the device is reported, with channel waveguides arranged in a Y-junction optical circuit, so that laser source stability requirements are lowered by a factor of 85 dB, making possible the use of low cost sources in practical applications. Molecular Diversity Preservation International (MDPI) 2008-11-11 /pmc/articles/PMC3787434/ /pubmed/27873918 http://dx.doi.org/10.3390/s8117113 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Parisi, Antonino Cino, Alfonso C. Busacca, Alessandro C. Cherchi, Matteo Riva-Sanseverino, Stefano
spellingShingle	Parisi, Antonino Cino, Alfonso C. Busacca, Alessandro C. Cherchi, Matteo Riva-Sanseverino, Stefano Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate
author_facet	Parisi, Antonino Cino, Alfonso C. Busacca, Alessandro C. Cherchi, Matteo Riva-Sanseverino, Stefano
author_sort	Parisi, Antonino
title	Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate
title_short	Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate
title_full	Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate
title_fullStr	Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate
title_full_unstemmed	Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate
title_sort	integrated optic surface plasmon resonance measurements in a borosilicate glass substrate
description	The surface plasmon resonance (SPR) technique is a well-known optical method that can be used to measure the refractive index of organic nano-layers adsorbed on a thin metal film. Although there are many configurations for measuring biomolecular interactions, SPR-based techniques play a central role in many current biosensing experiments, since they are the most suited for sensitive and quantitative kinetic measurements. Here we give some results from the analysis and numerical elaboration of SPR data from integrated optics experiments in a particular borosilicate glass, chosen for its composition offering the rather low refractive index of 1.4701 at 633 nm wavelength. These data regard the flow over the sensing region (metal window) of different solutions with refractive indexes in the range of interest (1.3÷1.5) for the detection of contaminants in aqueous solutions. After a discussion of the principles of SPR, of the metal window design optimization by means of optical interaction numerical modeling, and of waveguide fabrication techniques, we give a description of system setup and experimental results. Optimum gold film window thickness and width in this guided-wave configuration has been for the first time derived and implemented on an integrated optic prototype device. Its characterization is given by means of the real time waveguide output intensity measurements, which correspond to the interaction between the sensing gold thin film window and the flowing analyte. The SPR curve was subsequently inferred. Finally, a modified version of the device is reported, with channel waveguides arranged in a Y-junction optical circuit, so that laser source stability requirements are lowered by a factor of 85 dB, making possible the use of low cost sources in practical applications.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2008
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787434/
_version_	1612015385586958336

Integrated Optic Surface Plasmon Resonance Measurements in a Borosilicate Glass Substrate

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