Metagenomic Screening for Aromatic Compound-Responsive Transcriptional Regulators

We applied a metagenomics approach to screen for transcriptional regulators that sense aromatic compounds. The library was constructed by cloning environmental DNA fragments into a promoter-less vector containing green fluorescence protein. Fluorescence-based screening was then performed in the pres...

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Bibliographic Details
Main Authors: Uchiyama, Taku, Miyazaki, Kentaro
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786939/