Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line

Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogen...

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Main Authors:	Su, Feng, Liu, Xin, Liu, Guanghui, Yu, Yuan, Wang, Yongsheng, Jin, Yaping, Hu, Guangdong, Hua, Song, Zhang, Yong
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784436/

id	pubmed-3784436
recordtype	oai_dc
spelling	pubmed-37844362013-10-01 Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line Su, Feng Liu, Xin Liu, Guanghui Yu, Yuan Wang, Yongsheng Jin, Yaping Hu, Guangdong Hua, Song Zhang, Yong Research Article Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogenous immune research on bacterial invasion is necessary. In the present study, we first purified and immortalized type II AECs (AEC II cells) by transfecting them with a plasmid containing the human telomerase reverse trancriptase gene. We then tested whether or not the immortalized cells retained the basic physiological properties of primary AECs by reverse-transcription polymerase chain reaction and Western blot. Finally, we tested the secretion capacity of immortalized AEC II cells upon stimulation by bacterial invasion. The cattle type II alveolar epithelial cell line (HTERT-AEC II) that we established retained lung epithelial cell characteristics: the cells were positive for surfactants A and B, and they secreted tumor necrosis factor-α and interleukin-6 in response to bacterial invasion. Thus, the cell line we established is a potential tool for research on the relationship between AECs and Mycobacterium tuberculosis. Public Library of Science 2013-09-26 /pmc/articles/PMC3784436/ /pubmed/24086682 http://dx.doi.org/10.1371/journal.pone.0076036 Text en © 2013 su et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Su, Feng Liu, Xin Liu, Guanghui Yu, Yuan Wang, Yongsheng Jin, Yaping Hu, Guangdong Hua, Song Zhang, Yong
spellingShingle	Su, Feng Liu, Xin Liu, Guanghui Yu, Yuan Wang, Yongsheng Jin, Yaping Hu, Guangdong Hua, Song Zhang, Yong Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line
author_facet	Su, Feng Liu, Xin Liu, Guanghui Yu, Yuan Wang, Yongsheng Jin, Yaping Hu, Guangdong Hua, Song Zhang, Yong
author_sort	Su, Feng
title	Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line
title_short	Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line
title_full	Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line
title_fullStr	Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line
title_full_unstemmed	Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line
title_sort	establishment and evaluation of a stable cattle type ii alveolar epithelial cell line
description	Macrophages and dendritic cells are recognized as key players in the defense against mycobacterial infection. Recent research has confirmed that alveolar epithelial cells (AECs) also play important roles against mycobacterium infections. Thus, establishing a stable cattle AEC line for future endogenous immune research on bacterial invasion is necessary. In the present study, we first purified and immortalized type II AECs (AEC II cells) by transfecting them with a plasmid containing the human telomerase reverse trancriptase gene. We then tested whether or not the immortalized cells retained the basic physiological properties of primary AECs by reverse-transcription polymerase chain reaction and Western blot. Finally, we tested the secretion capacity of immortalized AEC II cells upon stimulation by bacterial invasion. The cattle type II alveolar epithelial cell line (HTERT-AEC II) that we established retained lung epithelial cell characteristics: the cells were positive for surfactants A and B, and they secreted tumor necrosis factor-α and interleukin-6 in response to bacterial invasion. Thus, the cell line we established is a potential tool for research on the relationship between AECs and Mycobacterium tuberculosis.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784436/
_version_	1612014627008282624

Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line

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