Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy
Objectives. To reveal the effect of microRNA-210 on cell apoptosis caused by HIE. Methods. Postnatal day 7 rats after HI injury were intraventricularly injected with microRNA-210 mimic, microRNA-210 inhibitor, or physiological saline. 72 h after the injection, rats were sacrificed and the left hemi...
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| Format: | Online |
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Hindawi Publishing Corporation
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782121/ |
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pubmed-3782121 |
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pubmed-37821212013-10-02 Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy Qiu, Jie Zhou, Xiao-yu Zhou, Xiao-guang Cheng, Rui Liu, Hai-ying Li, Yong Research Article Objectives. To reveal the effect of microRNA-210 on cell apoptosis caused by HIE. Methods. Postnatal day 7 rats after HI injury were intraventricularly injected with microRNA-210 mimic, microRNA-210 inhibitor, or physiological saline. 72 h after the injection, rats were sacrificed and the left hemispheres were collected. The expression level of microRNA-210 was identified by quantitative real-time PCR analysis. Apoptosis in brain sections was investigated by TUNEL assay. Apoptosis-related protein expressions were studied by Western blot analysis. Results. The results showed that microRNA-210, whose expression was downregulated in the brain 72 h after HI injury, suppressed neuronal apoptosis by inhibiting caspase activity and regulating the balance between bcl-2 and bax levels. Discussion. Recent study demonstrated that microRNA-210 has neuroprotective effects through inhibiting apoptosis in a murine model of HIE. It represents a potential novel therapeutic approach for the treatment of HIE. Hindawi Publishing Corporation 2013 2013-09-09 /pmc/articles/PMC3782121/ /pubmed/24089674 http://dx.doi.org/10.1155/2013/350419 Text en Copyright © 2013 Jie Qiu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Qiu, Jie Zhou, Xiao-yu Zhou, Xiao-guang Cheng, Rui Liu, Hai-ying Li, Yong |
| spellingShingle |
Qiu, Jie Zhou, Xiao-yu Zhou, Xiao-guang Cheng, Rui Liu, Hai-ying Li, Yong Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy |
| author_facet |
Qiu, Jie Zhou, Xiao-yu Zhou, Xiao-guang Cheng, Rui Liu, Hai-ying Li, Yong |
| author_sort |
Qiu, Jie |
| title |
Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy |
| title_short |
Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy |
| title_full |
Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy |
| title_fullStr |
Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy |
| title_full_unstemmed |
Neuroprotective Effects of MicroRNA-210 on Hypoxic-Ischemic Encephalopathy |
| title_sort |
neuroprotective effects of microrna-210 on hypoxic-ischemic encephalopathy |
| description |
Objectives. To reveal the effect of microRNA-210 on cell apoptosis caused by HIE. Methods. Postnatal day 7 rats after HI injury were intraventricularly injected with microRNA-210 mimic, microRNA-210 inhibitor, or physiological saline. 72 h after the injection, rats were sacrificed and the left hemispheres were collected. The expression level of microRNA-210 was identified by quantitative real-time PCR analysis. Apoptosis in brain sections was investigated by TUNEL assay. Apoptosis-related protein expressions were studied by Western blot analysis. Results. The results showed that microRNA-210, whose expression was downregulated in the brain 72 h after HI injury, suppressed neuronal apoptosis by inhibiting caspase activity and regulating the balance between bcl-2 and bax levels. Discussion. Recent study demonstrated that microRNA-210 has neuroprotective effects through inhibiting apoptosis in a murine model of HIE. It represents a potential novel therapeutic approach for the treatment of HIE. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782121/ |
| _version_ |
1612013975187226624 |