Examination of an absolute quantity of less than a hundred nanograms of proteins by amino acid analysis
We developed an ultra-sensitive method of amino acid analysis (AAA) for the absolute quantification of less than 100 ng of proteins, in solution or on polyvinylidene difluoride (PVDF) membranes using an oxygen-free chamber for protein hydrolysis. We used a pre-label method with 6-aminoquinolyl-N-hyd...
| Main Authors: | , |
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| Format: | Online |
| Language: | English |
| Published: |
Springer Berlin Heidelberg
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777156/ |