Visualizing SNVs to quantify allele-specific expression in single cells

We present a high efficiency fluorescence in situ hybridization method for detecting single nucleotide variants (SNVs) on individual RNA transcripts, both exonic and intronic. We used this method to quantify allelic expression at the population and single cell level, and also to distinguish maternal...

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Bibliographic Details
Main Authors: Levesque, Marshall J., Ginart, Paul, Wei, Yichen, Raj, Arjun
Format: Online
Language:English
Published: 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771873/