Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction
In tunnel construction, workers exposed to dust from blasting, gases, diesel exhausts, and oil mist have shown higher risk for pulmonary diseases. A clear mechanism to explain how these pollutants determine diseases is lacking, and alveolar epithelium's capacity to ingest inhaled fine particles...
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| Format: | Online |
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Hindawi Publishing Corporation
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771241/ |
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pubmed-3771241 |
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pubmed-37712412013-09-25 Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction Dominici, Luca Guerrera, Elena Villarini, Milena Fatigoni, Cristina Moretti, Massimo Blasi, Paolo Monarca, Silvano Research Article In tunnel construction, workers exposed to dust from blasting, gases, diesel exhausts, and oil mist have shown higher risk for pulmonary diseases. A clear mechanism to explain how these pollutants determine diseases is lacking, and alveolar epithelium's capacity to ingest inhaled fine particles is not well characterized. The objective of this study was to assess the genotoxic effect exerted by fine particles collected in seven tunnels using the cytokinesis-block micronuclei test in an in vitro model on type II lung epithelium A549 cells. For each tunnel, five fractions with different aerodynamic diameters of particulate matter were collected with a multistage cascade sampler. The human epithelial cell line A549 was exposed to 0.2 m3/mL equivalent of particulate for 24 h before testing. The cytotoxic effects of particulate matter on A549 cells were also evaluated in two different viability tests. In order to evaluate the cells' ability to take up fine particles, imaging with transmission electron microscopy of cells after exposure to particulate matter was performed. Particle endocytosis after 24 h exposure was observed as intracellular aggregates of membrane-bound particles. This morphologic evidence did not correspond to an increase in genotoxicity detected by the micronucleus test. Hindawi Publishing Corporation 2013 2013-08-28 /pmc/articles/PMC3771241/ /pubmed/24069598 http://dx.doi.org/10.1155/2013/345724 Text en Copyright © 2013 Luca Dominici et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Dominici, Luca Guerrera, Elena Villarini, Milena Fatigoni, Cristina Moretti, Massimo Blasi, Paolo Monarca, Silvano |
| spellingShingle |
Dominici, Luca Guerrera, Elena Villarini, Milena Fatigoni, Cristina Moretti, Massimo Blasi, Paolo Monarca, Silvano Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction |
| author_facet |
Dominici, Luca Guerrera, Elena Villarini, Milena Fatigoni, Cristina Moretti, Massimo Blasi, Paolo Monarca, Silvano |
| author_sort |
Dominici, Luca |
| title |
Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction |
| title_short |
Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction |
| title_full |
Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction |
| title_fullStr |
Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction |
| title_full_unstemmed |
Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction |
| title_sort |
evaluation of in vitro cytoxicity and genotoxicity of size-fractionated air particles sampled during road tunnel construction |
| description |
In tunnel construction, workers exposed to dust from blasting, gases, diesel exhausts, and oil mist have shown higher risk for pulmonary diseases. A clear mechanism to explain how these pollutants determine diseases is lacking, and alveolar epithelium's capacity to ingest inhaled fine particles is not well characterized. The objective of this study was to assess the genotoxic effect exerted by fine particles collected in seven tunnels using the cytokinesis-block micronuclei test in an in vitro model on type II lung epithelium A549 cells. For each tunnel, five fractions with different aerodynamic diameters of particulate matter were collected with a multistage cascade sampler. The human epithelial cell line A549 was exposed to 0.2 m3/mL equivalent of particulate for 24 h before testing. The cytotoxic effects of particulate matter on A549 cells were also evaluated in two different viability tests. In order to evaluate the cells' ability to take up fine particles, imaging with transmission electron microscopy of cells after exposure to particulate matter was performed. Particle endocytosis after 24 h exposure was observed as intracellular aggregates of membrane-bound particles. This morphologic evidence did not correspond to an increase in genotoxicity detected by the micronucleus test. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771241/ |
| _version_ |
1612011140053729280 |