Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O2.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with ei...
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The Korean Society for Parasitology and Tropical Medicine
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770882/ |
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pubmed-37708822013-09-13 Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis Song, Hyun-Ouk Ryu, Jae-Sook Brief Communication Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O2.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis. The Korean Society for Parasitology and Tropical Medicine 2013-08 2013-08-30 /pmc/articles/PMC3770882/ /pubmed/24039294 http://dx.doi.org/10.3347/kjp.2013.51.4.479 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Song, Hyun-Ouk Ryu, Jae-Sook |
| spellingShingle |
Song, Hyun-Ouk Ryu, Jae-Sook Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis |
| author_facet |
Song, Hyun-Ouk Ryu, Jae-Sook |
| author_sort |
Song, Hyun-Ouk |
| title |
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis |
| title_short |
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis |
| title_full |
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis |
| title_fullStr |
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis |
| title_full_unstemmed |
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis |
| title_sort |
superoxide anion production by human neutrophils activated by trichomonas vaginalis |
| description |
Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O2.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis. |
| publisher |
The Korean Society for Parasitology and Tropical Medicine |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770882/ |
| _version_ |
1612011079202766848 |