Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines

To advance the identification of quantitative trait loci (QTLs) to reduce Cd content in rice (Oryza sativa L.) grains and breed low-Cd cultivars, we developed a novel population consisting of 46 chromosome segment substitution lines (CSSLs) in which donor segments of LAC23, a cultivar reported to ha...

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Main Authors:	Abe, Tadashi, Nonoue, Yasunori, Ono, Nozomi, Omoteno, Motoyasu, Kuramata, Masato, Fukuoka, Shuichi, Yamamoto, Toshio, Yano, Masahiro, Ishikawa, Satoru
Format:	Online
Language:	English
Published:	Japanese Society of Breeding 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770555/

id	pubmed-3770555
recordtype	oai_dc
spelling	pubmed-37705552013-11-22 Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines Abe, Tadashi Nonoue, Yasunori Ono, Nozomi Omoteno, Motoyasu Kuramata, Masato Fukuoka, Shuichi Yamamoto, Toshio Yano, Masahiro Ishikawa, Satoru Articles To advance the identification of quantitative trait loci (QTLs) to reduce Cd content in rice (Oryza sativa L.) grains and breed low-Cd cultivars, we developed a novel population consisting of 46 chromosome segment substitution lines (CSSLs) in which donor segments of LAC23, a cultivar reported to have a low grain Cd content, were substituted into the Koshihikari genetic background. The parental cultivars and 32 CSSLs (the minimum set required for whole-genome coverage) were grown in two fields with different natural levels of soil Cd. QTL mapping by single-marker analysis using ANOVA indicated that eight chromosomal regions were associated with grain Cd content and detected a major QTL (qlGCd3) with a high F-test value in both fields (F = 9.19 and 5.60) on the long arm of chromosome 3. The LAC23 allele at qlGCd3 was associated with reduced grain Cd levels and appeared to reduce Cd transport from the shoots to the grains. Fine substitution mapping delimited qlGCd3 to a 3.5-Mbp region. Our results suggest that the low-Cd trait of LAC23 is controlled by multiple QTLs, and qlGCd3 is a promising candidate QTL to reduce the Cd level of rice grain. Japanese Society of Breeding 2013-09-01 2013-09 /pmc/articles/PMC3770555/ /pubmed/24273423 http://dx.doi.org/10.1270/jsbbs.63.284 Text en Copyright © 2013 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Abe, Tadashi Nonoue, Yasunori Ono, Nozomi Omoteno, Motoyasu Kuramata, Masato Fukuoka, Shuichi Yamamoto, Toshio Yano, Masahiro Ishikawa, Satoru
spellingShingle	Abe, Tadashi Nonoue, Yasunori Ono, Nozomi Omoteno, Motoyasu Kuramata, Masato Fukuoka, Shuichi Yamamoto, Toshio Yano, Masahiro Ishikawa, Satoru Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines
author_facet	Abe, Tadashi Nonoue, Yasunori Ono, Nozomi Omoteno, Motoyasu Kuramata, Masato Fukuoka, Shuichi Yamamoto, Toshio Yano, Masahiro Ishikawa, Satoru
author_sort	Abe, Tadashi
title	Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines
title_short	Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines
title_full	Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines
title_fullStr	Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines
title_full_unstemmed	Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines
title_sort	detection of qtls to reduce cadmium content in rice grains using lac23/koshihikari chromosome segment substitution lines
description	To advance the identification of quantitative trait loci (QTLs) to reduce Cd content in rice (Oryza sativa L.) grains and breed low-Cd cultivars, we developed a novel population consisting of 46 chromosome segment substitution lines (CSSLs) in which donor segments of LAC23, a cultivar reported to have a low grain Cd content, were substituted into the Koshihikari genetic background. The parental cultivars and 32 CSSLs (the minimum set required for whole-genome coverage) were grown in two fields with different natural levels of soil Cd. QTL mapping by single-marker analysis using ANOVA indicated that eight chromosomal regions were associated with grain Cd content and detected a major QTL (qlGCd3) with a high F-test value in both fields (F = 9.19 and 5.60) on the long arm of chromosome 3. The LAC23 allele at qlGCd3 was associated with reduced grain Cd levels and appeared to reduce Cd transport from the shoots to the grains. Fine substitution mapping delimited qlGCd3 to a 3.5-Mbp region. Our results suggest that the low-Cd trait of LAC23 is controlled by multiple QTLs, and qlGCd3 is a promising candidate QTL to reduce the Cd level of rice grain.
publisher	Japanese Society of Breeding
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770555/
_version_	1612010962106187776

Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines

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