Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis

Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to b...

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Main Authors:	Jin, Miao, Jiang, Yaoming, Sun, Linlin, Yin, Jianhua, Fu, Huihui, Wu, Genfu, Gao, Haichun
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769277/

id	pubmed-3769277
recordtype	oai_dc
spelling	pubmed-37692772013-09-13 Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis Jin, Miao Jiang, Yaoming Sun, Linlin Yin, Jianhua Fu, Huihui Wu, Genfu Gao, Haichun Research Article Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other γ-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation. Public Library of Science 2013-09-10 /pmc/articles/PMC3769277/ /pubmed/24040415 http://dx.doi.org/10.1371/journal.pone.0075610 Text en © 2013 Jin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jin, Miao Jiang, Yaoming Sun, Linlin Yin, Jianhua Fu, Huihui Wu, Genfu Gao, Haichun
spellingShingle	Jin, Miao Jiang, Yaoming Sun, Linlin Yin, Jianhua Fu, Huihui Wu, Genfu Gao, Haichun Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis
author_facet	Jin, Miao Jiang, Yaoming Sun, Linlin Yin, Jianhua Fu, Huihui Wu, Genfu Gao, Haichun
author_sort	Jin, Miao
title	Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis
title_short	Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis
title_full	Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis
title_fullStr	Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis
title_full_unstemmed	Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis
title_sort	unique organizational and functional features of the cytochrome c maturation system in shewanella oneidensis
description	Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other γ-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769277/
_version_	1612010638323744768

Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis

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