Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa

Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 25–1 experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2P...

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Main Authors:	Maldonade, Iriani R., Rodriguez-Amaya, Delia B., Scamparini, Adilma R. P.
Format:	Online
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768983/

id	pubmed-3768983
recordtype	oai_dc
spelling	pubmed-37689832013-09-12 Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa Maldonade, Iriani R. Rodriguez-Amaya, Delia B. Scamparini, Adilma R. P. Industrial Microbiology Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 25–1 experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract) was the most important variable in enhancing carotenoid production; MgSO4.7H2O and KH2PO4 had a negative influence. The initial pH had no significant effect on carotenoid and cell productions. We further investigated the effects of glucose and yeast extract effects, using a second-order central composite design (CCD) to optimise carotenoid production, which was adequately approximated with a full quadratic equation obtained from a two-factor-2-level design. The analysis of quadratic surfaces showed that after 5 days of cultivation at 25 °C, the maximum carotenoid concentration (745 µg l-1) was obtained with 15 g l-1 of yeast extract and 20 g l-1 of glucose. The maximum carotenoid production (152 µg g-1) was obtained with 5 g l-1 yeast extract and 10 g l-1 glucose. Carotenoid formation was more sensitive to changes in yeast extract concentration than to changes in glucose concentration. Maximum cell production was achieved with 15–17 g l-1 of yeast extract and 15–20 g l-1 of glucose. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768983/ /pubmed/24031809 http://dx.doi.org/10.1590/S1517-838220120001000012 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Maldonade, Iriani R. Rodriguez-Amaya, Delia B. Scamparini, Adilma R. P.
spellingShingle	Maldonade, Iriani R. Rodriguez-Amaya, Delia B. Scamparini, Adilma R. P. Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
author_facet	Maldonade, Iriani R. Rodriguez-Amaya, Delia B. Scamparini, Adilma R. P.
author_sort	Maldonade, Iriani R.
title	Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
title_short	Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
title_full	Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
title_fullStr	Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
title_full_unstemmed	Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
title_sort	statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa
description	Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 25–1 experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract) was the most important variable in enhancing carotenoid production; MgSO4.7H2O and KH2PO4 had a negative influence. The initial pH had no significant effect on carotenoid and cell productions. We further investigated the effects of glucose and yeast extract effects, using a second-order central composite design (CCD) to optimise carotenoid production, which was adequately approximated with a full quadratic equation obtained from a two-factor-2-level design. The analysis of quadratic surfaces showed that after 5 days of cultivation at 25 °C, the maximum carotenoid concentration (745 µg l-1) was obtained with 15 g l-1 of yeast extract and 20 g l-1 of glucose. The maximum carotenoid production (152 µg g-1) was obtained with 5 g l-1 yeast extract and 10 g l-1 glucose. Carotenoid formation was more sensitive to changes in yeast extract concentration than to changes in glucose concentration. Maximum cell production was achieved with 15–17 g l-1 of yeast extract and 15–20 g l-1 of glucose.
publisher	Sociedade Brasileira de Microbiologia
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768983/
_version_	1612010535580073984

Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa

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