A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens

Salmonella enterica serovar Gallinarum (SG) is a fowl typhoid agent in chickens and is a severe disease with worldwide economic impact as its mortality may reach up to 80%. It is one of a small group of serovars that typically produces typhoid-like infections in a narrow range of host species and wh...

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Main Authors:	de Paiva, Jacqueline Boldrin, Penha Filho, Rafael Antonio Casarin, Arguello, Yuli Melisa Sierra, Berchieri Junior, Ângelo, Lemos, Manuel Victor Franco, Barrow, Paul A.
Format:	Online
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2009
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768534/

id	pubmed-3768534
recordtype	oai_dc
spelling	pubmed-37685342013-09-12 A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens de Paiva, Jacqueline Boldrin Penha Filho, Rafael Antonio Casarin Arguello, Yuli Melisa Sierra Berchieri Junior, Ângelo Lemos, Manuel Victor Franco Barrow, Paul A. Veterinary Microbiology Salmonella enterica serovar Gallinarum (SG) is a fowl typhoid agent in chickens and is a severe disease with worldwide economic impact as its mortality may reach up to 80%. It is one of a small group of serovars that typically produces typhoid-like infections in a narrow range of host species and which therefore represents a good model for human typhoid. The survival mechanisms are not considered to be virulent mechanisms but are essential for the life of the bacterium. Mutants of Salmonella Gallinarum containing defective genes, related to cobalamin biosynthesis and which Salmonella spp. has to be produced to survive when it is in an anaerobic environment, were produced in this study. Salmonella Gallinarum is an intracellular parasite. Therefore, this study could provide information about whether vitamin B12 biosynthesis might be essential to its survival in the host. The results showed that the singular deletion in cbiA or cobS genes did not interfere in the life of Salmonella Gallinarum in the host, perhaps because single deletion is not enough to impede vitamin B12 biosynthesis. It was noticed that diluted SG mutants with single deletion produced higher mortality than the wild strain of SG. When double mutation was carried out, the Salmonella Gallinarum mutant was unable to provoke mortality in susceptible chickens. This work showed that B12 biosynthesis is a very important step in the metabolism of Salmonella Gallinarum during the infection of the chickens. Further research on bacterium physiology should be carried out to elucidate the events described in this research and to assess the mutant as a vaccine strain. Sociedade Brasileira de Microbiologia 2009 2009-09-01 /pmc/articles/PMC3768534/ /pubmed/24031393 http://dx.doi.org/10.1590/S1517-838220090003000012 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	de Paiva, Jacqueline Boldrin Penha Filho, Rafael Antonio Casarin Arguello, Yuli Melisa Sierra Berchieri Junior, Ângelo Lemos, Manuel Victor Franco Barrow, Paul A.
spellingShingle	de Paiva, Jacqueline Boldrin Penha Filho, Rafael Antonio Casarin Arguello, Yuli Melisa Sierra Berchieri Junior, Ângelo Lemos, Manuel Victor Franco Barrow, Paul A. A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
author_facet	de Paiva, Jacqueline Boldrin Penha Filho, Rafael Antonio Casarin Arguello, Yuli Melisa Sierra Berchieri Junior, Ângelo Lemos, Manuel Victor Franco Barrow, Paul A.
author_sort	de Paiva, Jacqueline Boldrin
title	A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
title_short	A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
title_full	A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
title_fullStr	A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
title_full_unstemmed	A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens
title_sort	defective mutant of salmonella enterica serovar gallinarum in cobalamin biosynthesis is avirulent in chickens
description	Salmonella enterica serovar Gallinarum (SG) is a fowl typhoid agent in chickens and is a severe disease with worldwide economic impact as its mortality may reach up to 80%. It is one of a small group of serovars that typically produces typhoid-like infections in a narrow range of host species and which therefore represents a good model for human typhoid. The survival mechanisms are not considered to be virulent mechanisms but are essential for the life of the bacterium. Mutants of Salmonella Gallinarum containing defective genes, related to cobalamin biosynthesis and which Salmonella spp. has to be produced to survive when it is in an anaerobic environment, were produced in this study. Salmonella Gallinarum is an intracellular parasite. Therefore, this study could provide information about whether vitamin B12 biosynthesis might be essential to its survival in the host. The results showed that the singular deletion in cbiA or cobS genes did not interfere in the life of Salmonella Gallinarum in the host, perhaps because single deletion is not enough to impede vitamin B12 biosynthesis. It was noticed that diluted SG mutants with single deletion produced higher mortality than the wild strain of SG. When double mutation was carried out, the Salmonella Gallinarum mutant was unable to provoke mortality in susceptible chickens. This work showed that B12 biosynthesis is a very important step in the metabolism of Salmonella Gallinarum during the infection of the chickens. Further research on bacterium physiology should be carried out to elucidate the events described in this research and to assess the mutant as a vaccine strain.
publisher	Sociedade Brasileira de Microbiologia
publishDate	2009
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768534/
_version_	1612010282072145920

A defective mutant of Salmonella enterica Serovar Gallinarum in cobalamin biosynthesis is avirulent in chickens

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