Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride
In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and...
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Sociedade Brasileira de Microbiologia
2008
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768419/ |
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pubmed-3768419 |
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pubmed-37684192013-09-12 Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride Matroudi, S. Zamani, M.R. Motallebi, M. Environmental Microbiology In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33 proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli. Sociedade Brasileira de Microbiologia 2008 2008-09-01 /pmc/articles/PMC3768419/ /pubmed/24031242 http://dx.doi.org/10.1590/S1517-83822008000300005 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Matroudi, S. Zamani, M.R. Motallebi, M. |
| spellingShingle |
Matroudi, S. Zamani, M.R. Motallebi, M. Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride |
| author_facet |
Matroudi, S. Zamani, M.R. Motallebi, M. |
| author_sort |
Matroudi, S. |
| title |
Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride |
| title_short |
Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride |
| title_full |
Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride |
| title_fullStr |
Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride |
| title_full_unstemmed |
Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride |
| title_sort |
molecular cloning of chitinase 33 (chit33) gene from trichoderma atroviride |
| description |
In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33 proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli. |
| publisher |
Sociedade Brasileira de Microbiologia |
| publishDate |
2008 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768419/ |
| _version_ |
1612010217634004992 |